• Product Nameα-tubulin Mouse Monoclonal Antibody
• Host SpeciesMouse
• ClonalityMonoclonal
• PurificationAffinity purification using immunogen.
• ApplicationsWB,IHC,IF
• Species ReactivityHu Rt Ms
• SpecificityThe α-tubulin antibody can detects endogenousα-tubulin protein.
• Target Nameα-tubulin
• Alternative Names    TUBA1A;TUBA3;Tubulin alpha-1A chain;Alpha-tubulin 3;Tubulin B-alpha-1;Tubulin alpha-3 chain
• Accession No. Swiss-Prot:Q71U36
  Gene ID:7846
• UniprotQ71U36
• Gene ID7846;
• SDS-PAGE MW 52kd
• Concentration 1.0mg/ml
• Formulation Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB dilution: 1:5000~1:10000
  IHC dilution:1:200 IF dilution: 1:100~1:200
  IP dilution: 1:200

Tubulin is one of several members of a small family of globular proteins. The tubulin superfamily includes five distinct families, the alpha-, beta-, gamma-, delta-, and epsilon-tubulins. The most common members of the tubulin family are α-tubulin and β-tubulin, the proteins that make up microtubules. Each has a molecular weight of approximately 55 KD. Microtubules are assembled from dimers of α- and β-tubulin

• Western blot analysis of 1) Hela, 2) Rat BrianTissue, 3) Mouse Brain Tissue, using #37981 diluted at 1:5,000.
• IF analysis of?Hela?with #37981(Left) and DAPI (Right) diluted at 1:100.
• 1¡¢Input: Mouse Brain Tissue Lysate
  2¡¢IP product: IP dilute 1:200
  Western blot analysis: primary antibody : #37981 1:5,000
  Secondary antibody: Goat anti-Mouse IgG, Light chain specific, 1:5,000
• Immunofluorescence analysis of Human-colon-cancer tissue. 1,-tubulin Monoclonal Antibody(8F11)(red) was diluted at 1:200(4C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
• Immunofluorescence analysis of Mouse-liver tissue. 1,-tubulin Monoclonal Antibody(8F11)(red) was diluted at 1:200(4C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
• Immunohistochemical analysis of paraffin-embedded Human-uterus-cancer tissue. 1,-tubulin Monoclonal Antibody(8F11) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
• Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1,-tubulin Monoclonal Antibody(8F11) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
• Immunohistochemical analysis of paraffin-embedded Mouse-heart tissue. 1,-tubulin Monoclonal Antibody(8F11) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting