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Histone H3 Mouse Monoclonal Antibody

37988
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  Catalog No. Package Size Price
37988 100ul $279.00

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Description
  • Product Name Histone H3 Mouse Monoclonal Antibody
  • Host Species Mouse
  • Clonality Monoclonal
  • Purification Affinity purification using immunogen.
  • Applications WB IF IP
  • Species Reactivity Hu Rt Ms
  • Specificity The Histone H3 antibody can detects endogenous Histone H3 protein.
  • Target Name Histone H3
  • Alternative Names H3/A; H3FA;H3FB; H3FC
  • Accession No. Swiss-Prot#:P68431
  • SDS-PAGE MW 17kd
  • Concentration 1.0mg/ml
  • Formulation Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage Store at -20˚C
Application Details

Western blotting: 1:2000~1:5000
Immunofluorescence: 1:100-500
Immunoprecipitation: 1:200

Images
  • Histone H3 Mouse Monoclonal Antibody - SAB | Signalway Antibody

    Western blot analysis of 1) Hela, 2) Raw, 3) Mouse Brain Tissue, 4) Rat Brain Tissue, using #37988 diluted at 1:5,000.

  • Histone H3 Mouse Monoclonal Antibody - SAB | Signalway Antibody

    IF analysis of Hela with DAPI (Left) and #37988(Right) diluted at 1:200.

  • Histone H3 Mouse Monoclonal Antibody - SAB | Signalway Antibody

    1¡¢Input: Hela Cell Lysate
    2¡¢IP product: IP dilute 1:200
    Western blot analysis: primary antibody : #37988 1:2,000
    Secondary antibody: Goat anti-Mouse IgG(H+L) 1:10,000.

Background
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.
    Please let us know if you have published research using #37988 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  
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