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β II tubulin Mouse Monoclonal Antibody (Rodent Preferred)

38092
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  Catalog No. Package Size Price
38092 100ul $279.00

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Description
  • Product Name β II tubulin Mouse Monoclonal Antibody (Rodent Preferred)
  • Host Species Mouse
  • Clonality Monoclonal
  • Purification Affinity purification using immunogen.
  • Applications WB,IHC
  • Species Reactivity Rt Ms
  • Specificity The β II tubulin Mouse Monoclonal Antibody detects endogenous β II tubulin protein.
  • Target Name β II tubulin
  • SDS-PAGE MW 50kd
  • Concentration 1.0mg/ml
  • Formulation Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage Store at -20˚C
Application Details

WB dilution: 1:50000~1:100000
IHC dilution:1:50-300
IP dilution: 1:200

Images
  • β II tubulin Mouse Monoclonal Antibody (Rodent Preferred) - SAB | Signalway Antibody

    Western blot analysis of 1) Mouse Brain tissue, 2) Rat Brain tissue, using #38092 diluted at 1:100,000.

  • β II tubulin Mouse Monoclonal Antibody (Rodent Preferred) - SAB | Signalway Antibody

    1¡¢Input: Mouse Brain Tissue Lysate
    2¡¢IP product: IP dilute 1:200
    Western blot analysis: primary antibody : #38092 1:10,000
    Secondary antibody: Goat anti-Mouse IgG, Light chain specific, 1:5,000

  • β II tubulin Mouse Monoclonal Antibody (Rodent Preferred) - SAB | Signalway Antibody

    Immunohistochemical analysis of paraffin-embedded Human-colon tissue. 1, II tubulin Monoclonal Antibody(Mix) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

  • β II tubulin Mouse Monoclonal Antibody (Rodent Preferred) - SAB | Signalway Antibody

    Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1, II tubulin Monoclonal Antibody(Mix) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

  • β II tubulin Mouse Monoclonal Antibody (Rodent Preferred) - SAB | Signalway Antibody

    Immunohistochemical analysis of paraffin-embedded Mouse-testis tissue. 1, II tubulin Monoclonal Antibody(Mix) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

Background
Tubulin is the major constituent of microtubules. It exists as a heterodimer consiting of an alpha and a beta subunit. Class II beta tubulin is the major form of tubulin beta in neurons, although it is not neuronal specific. It is found in many other tissues including lung tissue and Schwann cells.
    Please let us know if you have published research using #38092 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  
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