Your Position: Home > Antibodies > HIST4H4 antibody

HIST4H4 antibody

38198
Review: comment rank 5 pdf
  Catalog No. Package Size Price
38198 100ul $279.00

Quantity:  Total:   
 

Availability: promotionThe lead time is currently 2 weeks.

add promotion
Description
  • Product Name HIST4H4 antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Purification Antibodies were purified by affinity purification using immunogen.
  • Applications WB
  • Species Reactivity Hu Ms Rt
  • Specificity The antibody detects endogenous level of total HIST4H4 antibody.
  • Immunogen Type Recombinant Protein
  • Immunogen Description Recombinant protein of human HIST4H4.
  • Target Name HIST4H4
  • Alternative Names HIST4H4;H4/p;HIST1H4A;HIST1H4B;HIST1H4C;HIST1H4D;HIST1H4E;HIST1H4F;HIS T1H4H;HIST1H4I;HIST1H4J;HIST1H4K;HIST1H4L;HIST2H4A;HIST2H4B;MGC24116;Histone H4;
  • Accession No. Swiss-Prot#: P62805
    NCBI Gene ID: 121504
  • SDS-PAGE MW 11kd
  • Concentration 1.0mg/ml
  • Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage Store at -20˚C
Application Details

Western blotting: 1:500 - 1:2000

Images
  • HIST4H4 antibody - SAB | Signalway Antibody

    Western blot analysis of extracts of HeLa cell line, using HIST4H4 antibody.

Background

The nucleosome, made up of four core histone proteins (H2A, H2B, H3, and H4), is the primary building block of chromatin. Originally thought to function as a static scaffold for DNA packaging, histones have now been shown to be dynamic proteins, undergoing multiple types of post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (1,2). Histone acetylation occurs mainly on the amino-terminal tail domains of histones H2A (Lys5), H2B (Lys5, 12, 15, and 20), H3 (Lys9, 14, 18, 23, 27, and 56), and H4 (Lys5, 8, 12, and 16) and is important for the regulation of histone deposition, transcriptional activation, DNA replication, recombination, and DNA repair (1-3). Hyper-acetylation of the histone tails neutralizes the positive charge of these domains and is believed to weaken histone-DNA and nucleosome-nucleosome interactions, thereby destabilizing chromatin structure and increasing the accessibility of DNA to various DNA-binding proteins (4,5). In addition, acetylation of specific lysine residues creates docking sites for a protein module called the bromodomain, which binds to acetylated lysine residues (6). Many transcription and chromatin regulatory proteins contain bromodomains and may be recruited to gene promoters, in part, through binding of acetylated histone tails. Histone acetylation is mediated by histone acetyltransferases (HATs), such as CBP/p300, GCN5L2, PCAF, and Tip60, which are recruited to genes by DNA-bound protein factors to facilitate transcriptional activation (3). Deacetylation, which is mediated by histone deacetylases (HDAC and sirtuin proteins), reverses the effects of acetylation and generally facilitates transcriptional repression (7,8). Histone H4 lysine 5 is acetylated by multiple HAT proteins. Acetylation by Esa1p in yeast, or Tip60 in mammalian cells, may contribute to both transcriptional activation and DNA repair, including non-homologous end joining and replication-coupled repair (9-12).

    Please let us know if you have published research using #38198 so that we can cite your reference.
Customer Reviews and Rankings
  • No comment
  • Total 0 results, divided into1 pages. First Prev Next Last

    My Review

    Username: Anonymous user
    E-mail:
    Ratings:
    Content:
    Verification code: captcha
     
    Protocol
    Browsing historyclear
    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  
      SUBSCRIBE OUR E-MAIL
    • Inspiration, ideas and your feedback.
    FOLLOW US

    facebook twitter linkedin

    sab © 2017 Signalway Antibody LLC. All Rights Reserved.  E-mail: info@signalwayantibody.com