• Product NamePF-477736
• Brief DescriptionInhibitors
• Purification98.34%
• Biological ActivityPF-477736 is a selective, potent and ATP-competitive Chk1 inhibitor with Ki of 0.49 nM and also inhibits VEGFR2, Aurora-A, FGFR3, Flt3, Fms (CSF1R), Ret and Yes. It shows ~100-fold selectivity for Chk1 than Chk2. Phase 1.
• Target Namec-Fms inhibitor; Chk inhibitor; Src inhibitor; VEGFR inhibitor
• CAS No. 952021-60-2
• Calculated MW 419.48
• Formulation C22H25N7O2
• Storage 3 years -20˚C powder;2 years -80˚C in solvent;

• Research Area:Cancer
  SMILES:CN1C=C(C=N1)C2=NC3=C4C2=CNNC(=O)C4=CC(=C3)NC(=O)[C@@H](C5CCCCC5)N
  Pathways:Angiogenesis; Cell Cycle/Checkpoint; Tyrosine Kinase/Adaptors
  Receptor:c-Fms; Chk1; Chk2; Yes; VEGFR2
  Boling pt:
  Melting pt:
  Solubility:DMSO:6 mg/mL (14.3 mM)Ethanol:<1 mg/mL (<1 mM)Water:<1 mg/mL (<1 mM)
  Appearance:
  Remark:For obtaining a higher solubility , please warm the tube at 37 ˚C and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20 ˚C for several month.

1. Blasina A, et al. Mol Y Ther, 2008, 7(8), 2394-2404.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting