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Hydroxyproline (Digestion Method) Assay Kit

CA147
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  Catalog No. Package Size Price
CA147 50T/48 samples $199.00

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Description
  • Product Name Hydroxyproline (Digestion Method) Assay Kit
  • Brief Description Colorimetry
  • Applications Biochemical Assay
Application Details

No. Parameter name Parameters 1 Detection limit 0.01g/ml 2 CV in a batch 2.2% 3 CV between batchs 4.65% 4 Recovery rate 102% 5 Linear range: 0-20g/ml R2=0.9998 6 Chromogenic stability All steps should be done in 1 hour.

Product Description
Introduction:This kit applies to measure hydroxyproline content in various samples such as blood serum, body fluids, tissue homogenates, cell culture fluid, cultured cells, etc. This kit can be used for laboratory research only.
Significance:2. Assay significance: Collagen protein contains 13.4% hydroxyproline, elastin contains thimbleful hydroxyproline, other proteins contain no hydroxyproline. Collagen protein mainly exists in skin, tendon, cartilage, vessels, etc. As result, hydroxyproline content can reflect collagen metabolic state in connective tissue diseases. Some researchers consider skin hydroxyproline assay as a factor of anti-aging medicine selection. In growth period, hydroxyproline content in urine increases while grow up, hydroxyproline content keeps stable in adult period, it decrease in senescence phase. When rat/mouse suffers essential or secondary hypertension, hydroxyproline conten in urine will increase. Operation wound can cause urine hydroxyproline excretion increasing. As result of hyperlipemia, protein shortage, alcoholism, toxin, malnutrition, or degeneration & necrosis of liver cells, fiber increases by inflammation, dissectes hepatic lobules and leads to hepatic fibrosis. During hepatic fibrosis development, main increasing matter is collagen protein, collagen contains hydroxyproline. So it is able to calculate liver collagen content and reflect hepatic fibrosis level by measuring hydroxyproline content. Collagen fiber decomposition mainly depends on collagenase & other proteases, its decomposed product hydroxyproline is excreted in urine. So it is able to reflect collagen degradation by measuring hydroxyproline content in urine. Hydroxyproline assay in tissue & urine can be used to determine fibrosis level and select medicine of prophylaxis & heal.
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