• Product NamePhospho-JNK1/2/3(T183+T183+T221) Rabbit mAb
• Host SpeciesRabbit
• ClonalityMonoclonal
• Clone No.ST500
• PurificationProA affinity purified
• ApplicationsWB, ICC/IF, IHC, IP, FC
• Species ReactivityHu, Ms, Rt
• Immunogen DescriptionSynthetic phospho-peptide corresponding to residues surrounding Thr183 + Thr183 + Thr221 of human JNK1/2/3
• Alternative Names    C Jun kinase 2 antibody
  c Jun N terminal kinase 1 antibody
  c Jun N terminal kinase 2 antibody
  c Jun N terminal kinase 3 antibody
  c-Jun N-terminal kinase 1 antibody
  JNK 46 antibody
  JNK 55 antibody
  JNK antibody
  JNK-46 antibody
  JNK1 antibody
  JNK1A2 antibody
  JNK2 antibody
  JNK21B1/2 antibody
  JNK2A antibody
  JNK2ALPHA antibody
  JNK2B antibody
  JNK2BETA antibody
  JNK3 alpha protein kinase antibody
  JNK3 antibody
  JNK3A antibody
  Jun kinase antibody
  JUN N terminal kinase antibody
  MAP kinase 10 antibody
  MAP kinase 8 antibody
  MAP kinase 9 antibody
  MAP kinase p49 3F12 antibody
  MAPK 10 antibody
  MAPK 8 antibody
  MAPK 9 antibody
  MAPK10 antibody
  mapk8 antibody
  MAPK9 antibody
  Mitogen activated protein kinase 10 antibody
  Mitogen activated protein kinase 8 antibody
  Mitogen activated protein kinase 8 isoform JNK1 alpha1 antibody
  Mitogen activated protein kinase 8 isoform JNK1 beta2 antibody
  Mitogen activated protein kinase 9 antibody
  Mitogen-activated protein kinase 8 antibody
  MK08_HUMAN antibody
  p493F12 antibody
  p54a antibody
  p54aSAPK antibody
  p54bSAPK antibody
  PRKM10 antibody
  PRKM8 antibody
  PRKM9 antibody
  SAPK antibody
  SAPK(beta) antibody
  SAPK1 antibody
  SAPK1a antibody
  SAPK1b antibody
  SAPK1c antibody
  Stress activated protein kinase 1 antibody
  Stress activated protein kinase 1a antibody
  Stress activated protein kinase 1b antibody
  Stress activated protein kinase 1c antibody
  Stress activated protein kinase beta antibody
  Stress activated protein kinase JNK1 antibody
  Stress activated protein kinase JNK2 antibody
  Stress activated protein kinase JNK3 antibody
  Stress-activated protein kinase 1c antibody
  Stress-activated protein kinase JNK1 antibody
  
• Accession No. Swiss-Prot#:P45983
• UniprotP45983
• Gene ID5599;
• Calculated MW 54/46 kDa
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:1,000-1:2,000
  IHC: 1:50-1:200
  ICC: 1:50-1:200
  
  FC: 1:50-1:100

JNKs (c-Jun N-terminal kinases) belong to a family of MAP kinases that are involved in a variety of cellular processes, including transcriptional regulation and cellular proliferation, differentiation and development. JNK2 (c-Jun N-terminal kinase 2) and JNK3 (c-Jun N-terminal kinase 3) are 424 and 464 amino acid proteins, respectively, that each contain one protein kinase domain and use magnesium as a cofactor to catalyze the phosphorylation of target proteins, thereby playing a role in a variety of events throughout the cell. Both JNK2 and JNK3 exist as multiple alternatively spliced isoforms and are subject to post-translational phosphorylation on Thr 183 and Thr 221, respectively, an event which activates JNK2/JNK3 enzymatic activity. Defects in the gene encoding JNK3 are a cause of epileptic encephalopathy of the Lennox-Gastaut type, a group of epileptic disorders characterized by severe psychomotor delay and seizures.

• Western blot analysis of Phospho-JNK1/2/3(T183+T183+T221) on different lysates using anti-Phospho-JNK1/2/3(T183+T183+T221) antibody at 1/1,000 dilution. Positive control:
  Lane 1: NIH/3T3 cell lysate, treated with Anisomycin
  Lane 2: NIH/3T3 cell lysate, untreated
• Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Phospho-JNK1/2/3(T183+T183+T221) antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-Phospho-JNK1/2/3(T183+T183+T221) antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-Phospho-JNK1/2/3(T183+T183+T221) antibody. Counter stained with hematoxylin.
• ICC staining Phospho-JNK1/2/3(T183+T183+T221) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining Phospho-JNK1/2/3(T183+T183+T221) in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining Phospho-JNK1/2/3(T183+T183+T221) in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• Flow cytometric analysis of Hela cells with Phospho-JNK1/2/3(T183+T183+T221) antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting