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Human Pleural Effusion Tumor Cell MACS Kit

MK001
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  Catalog No. Package Size Price
MK001 10T $419.00

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Description
  • Product Name Human Pleural Effusion Tumor Cell MACS Kit
  • Brief Description Immunomagnetic beads capture liquid biopsy method
  • Applications MACS
  • Species Reactivity Hu
  • Alternative Names Specific Protein 70 ; SP70; TSP70
  • Accession No. NCBI GEO: GSE51947
  • Storage 1.Storage conditions: 2 ~ 8 ℃. 2. Expiration Date: 12 months after the production date. Reagents should be immediately put back at 2-8℃ after openning. Avoid freezing. Suggest to be used in one month after openning.
Application Details

Standard configuration microscopy or digital pathological slide scanners are recommended.

Sample requirements
1. The amount of pleural effusion required for this test shall be no less than 10ml.
2. The pleural effusion after the acquisition, placed no more than 24 hours at room temperature, 2 ℃ ~ 8 ℃ placed no more than 4 days.

Product Description

Intended use: This kit selects tumor cells expressing SP70 in pleural effusion positively and selects tumor cells from occlusion cells, eliminating the interference of a large number of occlusion cells in pleural effusion.The immunomagnetic bead antibodies provided by this kit are mouse monoclonal antibody.

Detection principle: Immunomagnetic beads coated with monoclonal antibody are incubated with pleural effusion samples, and the tumor cells expressing SP70 antigen identified by monoclonal antibody are captured by magnetic force to achieve the purpose of isolating tumor cells in pleural effusion. Further microscopy was performed after pasteurization to determine the pathological nature of pleural exfoliated cells. In the quality control, it is recommended to use the established lung adenocarcinoma cells as the positive control and the healthy human blood cells as the negative control.

Regerences

 Pan S, Wang F, Huang P, et al. The Study on Newly Developed McAb NJ001 Specific to non-small Cell Lung Cancer and Its Biological Characteristics. PLoS ONE, 2012,7(3):e33009<br />   [Patent] Monoclonal antibody against human non-small cell lung carcinoma and use thereof (US 8,911,727 B2)

    Please let us know if you have published research using #MK001 so that we can cite your reference.
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      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
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    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
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