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Mouse Anti-Human GL50 (B7-H2) mAb

CM035
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  Catalog No. Package Size Price
CM035-1 25ug $159.00
CM035-2 100ug $478.00

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Description
  • Product Name Mouse Anti-Human GL50 (B7-H2) mAb
  • Host Species Mouse
  • Clonality Monoclonal
  • Clone No. 20B10
  • Isotype Mouse IgG1, κ
  • Applications FC/ELISA
  • Species Reactivity Hu
  • Specificity This antibody recognizes human B7-H2 in FACS.
  • Immunogen Description L929/B7-H2 transfected cells
  • Alternative Names ICOS Ligand , B7-H2 , ICOSLG
  • Formulation Lyophilized from a 0.2μm filtered solution in phosphate buffered saline (PBS) and reconstitute with sterile PBS .
  • Storage Store protected from light at 2-8˚C. Do not freeze. The expiration date is indicated on the vial label.
Application Details

Preparation:This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a mouse immunized with L929/B7-H2 transfected cells). The monoclonal antibody was purified from tissue culture supernatant or ascites by protein G affinity chromatography.
Product Notices:This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 10^6 cells in a 100-?l experimental sample (per test). An isotype control should be used at the same concentration as the antibody of interest.

Images
  • Mouse Anti-Human GL50 (B7-H2) mAb - SAB | Signalway Antibody

    Flow cytometric analysis of ICOSL expression on L929/ICOSL transfected cells. L929/ICOSL transfected cells were stained with either Mouse IgG2a, κ Isotype Control or Mouse Anti-Human ICOSL antibody. L929/B7-H4 transfected cells and L929/Mock cells were stained with Mouse Anti-Human ICOSL antibody respectively. Fluorescence histograms showing the expression of ICOSL (or Ig Isotype control staining) were derived from events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a Beckman FC 500 Flow Cytometer System

Product Description
Inducible co-stimulator ligand (ICOSL), also known as B7-H2, is a member of the B7 family of co-stimulatory molecules related to B7-1 and B7-2. It is a transmembrane glycoprotein with extracellular IgV and IgC domains, and binds to ICOS on activated T cells, thus delivers a positive costimulatory signal for optimal T cell function. The structural features of ICOSL are crucial for its costimulatory function. Some studies show that ICOSL displays a marked oligomerization potential, resembling more like B7-1 than B7-2. B7-H2-dependent signaling may play an active role in a proliferative response rather than in cytokine and chemokine production. The CD28/B7 and ICOS/B7-H2 pathways are both critical for costimulating T cell immune responses. Deficiency in either pathway results in defective T cell activation, cytokine production and germinal center formation.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  
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