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EGFR (Phospho-Ser1026) Conjugated Antibody

C11902
Review: comment rank 5 pdf
  Catalog No. Package Size Price
C11902-AF488 100ul $379.00
C11902-AF594 100ul $379.00
C11902-AF647 100ul $379.00
C11902-AF790 100ul $399.00
C11902-Biotin 100ul $359.00
C11902-Cy3 100ul $379.00
C11902-Cy5 100ul $379.00

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Description
  • Product Name EGFR (Phospho-Ser1026) Conjugated Antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Species Reactivity Hu
  • Specificity The antibody detects endogenous level of EGFR only when phosphorylated at serine 1026.
  • Immunogen Description Peptide sequence around phosphorylation site of serine 1026 (P-S-S(p)-P-S) derived from Human EGFR.
  • Alternative Names ERBB1;Epidermal growth factor receptor precursor;kinase EGFR
  • Accession No. Swiss-Prot#:P00533
    NCBI Gene ID:1956
    NCBI mRNA#:NM_005228.3
    NCBI Protein#: NP_005219.2
  • Calculated MW 160
  • Concentration
  • Formulation 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 10mg/ml Bovine Serum Albumin, 0.05% Sodium Azide
  • Storage Store at 4℃ in dark for 6 months
Application Details

Biotin conjugated: Working with enzyme-conjugated streptavidin ELISA: 1: 200 - 1: 1,000 Western Blot: 1: 200 - 1: 1,000 IHC/ICC: 1: 100 - 1: 500 Flow Cytometry: 1: 50 - 1: 250 IF: 1: 100 - 1: 500 AF488 conjugated: most apllications: 1: 50 - 1: 250 AF594 conjugated: most apllications: 1: 50 - 1: 250 AF647 conjugated: most apllications: 1: 50 - 1: 250 AF790 conjugated: Western blot: 1: 100-1,000 Cy3 conjugated: most apllications: 1: 50 - 1: 250 Cy5 conjugated: most apllications: 1: 50 - 1: 250
desc_detail:Excitation/Emission Alexa Fluor 488 (AF488): 493nm/519nm Alexa Fluor 594 (AF594): 591nm/614nm Alexa Fluor 647 (AF647): 651nm/667nm Alexa Fluor 790 (AF790): 792nm/803nm Cyanine Cy3 (Cy3): 550nm/570nm Cyanine Cy5 (Cy5): 650nm/670nm

Product Description
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Background
Receptor for EGF, but also for other members of the EGF family, as TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Is involved in the control of cell growth and differentiation. Phosphorylates MUC1 in breast cancer cells and increases the interaction of MUC1 with SRC and CTNNB1/beta-catenin.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  
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