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PDGFRb (Phospho-Tyr771) Conjugated Antibody

C11907
Review: comment rank 5 pdf
  Catalog No. Package Size Price
C11907-AF488 100ul $379.00
C11907-AF594 100ul $379.00
C11907-AF647 100ul $379.00
C11907-AF790 100ul $399.00
C11907-Biotin 100ul $359.00
C11907-Cy3 100ul $379.00
C11907-Cy5 100ul $379.00

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Description
  • Product Name PDGFRb (Phospho-Tyr771) Conjugated Antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Species Reactivity Hu
  • Specificity The antibody detects endogenous level of PDGFRb only when phosphorylated at tyrosine 771.
  • Immunogen Description Peptide sequence around phosphorylation site of tyrosine 771 (S-N-Y(p)-M-A) derived from Human PDGFRb.
  • Alternative Names CD140b;PDGF-R-beta;PDGFR;PGFRB;kinase PDGFR-beta
  • Accession No. Swiss-Prot#:P09619
    NCBI Gene ID:5159
    NCBI mRNA#:NM_002609.3
    NCBI Protein#: NP_002600.1
  • Calculated MW 190
  • Concentration
  • Formulation 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 10mg/ml Bovine Serum Albumin, 0.05% Sodium Azide
  • Storage Store at 4℃ in dark for 6 months
Application Details

Biotin conjugated: Working with enzyme-conjugated streptavidin ELISA: 1: 200 - 1: 1,000 Western Blot: 1: 200 - 1: 1,000 IHC/ICC: 1: 100 - 1: 500 Flow Cytometry: 1: 50 - 1: 250 IF: 1: 100 - 1: 500 AF488 conjugated: most apllications: 1: 50 - 1: 250 AF594 conjugated: most apllications: 1: 50 - 1: 250 AF647 conjugated: most apllications: 1: 50 - 1: 250 AF790 conjugated: Western blot: 1: 100-1,000 Cy3 conjugated: most apllications: 1: 50 - 1: 250 Cy5 conjugated: most apllications: 1: 50 - 1: 250
desc_detail:Excitation/Emission Alexa Fluor 488 (AF488): 493nm/519nm Alexa Fluor 594 (AF594): 591nm/614nm Alexa Fluor 647 (AF647): 651nm/667nm Alexa Fluor 790 (AF790): 792nm/803nm Cyanine Cy3 (Cy3): 550nm/570nm Cyanine Cy5 (Cy5): 650nm/670nm

Product Description
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Background
PDGF Receptor β encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. This gene is flanked on chromosome 5 by the genes for granulocyte-macrophage colony-stimulating factor and macrophage-colony stimulating factor receptor; all three genes may be implicated in the 5-q syndrome. A translocation between chromosomes 5 and 12, that fuses this gene to that of the translocation, ETV6, leukemia gene, results in chronic myeloproliferative disorder with eosinophilia.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  
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