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Ezrin (Phospho-Tyr146) Conjugated Antibody

C11909
Review: comment rank 5 pdf
  Catalog No. Package Size Price
C11909-AF488 100ul $379.00
C11909-AF594 100ul $379.00
C11909-AF647 100ul $379.00
C11909-AF790 100ul $399.00
C11909-Biotin 100ul $359.00
C11909-Cy3 100ul $379.00
C11909-Cy5 100ul $379.00

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Description
  • Product Name Ezrin (Phospho-Tyr146) Conjugated Antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Species Reactivity Hu
  • Specificity The antibody detects endogenous level of Ezrin only when phosphorylated at tyrosine 146.
  • Immunogen Description Peptide sequence around phosphorylation site of tyrosine 146 (S-G-Y(p)-L-S) derived from Human Ezrin.
  • Alternative Names Cytovillin;EZRI;p81;VIL2;Villin 2
  • Accession No. Swiss-Prot#:P15311
    NCBI Gene ID:7430
    NCBI mRNA#:NM_001111077.1
    NCBI Protein#: NP_001104547.1
  • Calculated MW 69
  • Concentration
  • Formulation 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 10mg/ml Bovine Serum Albumin, 0.05% Sodium Azide
  • Storage Store at 4℃ in dark for 6 months
Application Details

Biotin conjugated: Working with enzyme-conjugated streptavidin ELISA: 1: 200 - 1: 1,000 Western Blot: 1: 200 - 1: 1,000 IHC/ICC: 1: 100 - 1: 500 Flow Cytometry: 1: 50 - 1: 250 IF: 1: 100 - 1: 500 AF488 conjugated: most apllications: 1: 50 - 1: 250 AF594 conjugated: most apllications: 1: 50 - 1: 250 AF647 conjugated: most apllications: 1: 50 - 1: 250 AF790 conjugated: Western blot: 1: 100-1,000 Cy3 conjugated: most apllications: 1: 50 - 1: 250 Cy5 conjugated: most apllications: 1: 50 - 1: 250
desc_detail:Excitation/Emission Alexa Fluor 488 (AF488): 493nm/519nm Alexa Fluor 594 (AF594): 591nm/614nm Alexa Fluor 647 (AF647): 651nm/667nm Alexa Fluor 790 (AF790): 792nm/803nm Cyanine Cy3 (Cy3): 550nm/570nm Cyanine Cy5 (Cy5): 650nm/670nm

Product Description
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Background
Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis.
    Please let us know if you have published research using #C11909 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  
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