• Product NameIRF-3 (phospho Ser396) Polyclonal Antibody
• Host SpeciesRabbit
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB,ELISA
• Species ReactivityHuman,Mouse,Rat
• SpecificityPhospho-IRF-3 (S396) Polyclonal Antibody detects endogenous levels of IRF-3 protein only when phosphorylated at S396.
• Immunogen DescriptionThe antiserum was produced against synthesized peptide derived from human IRF-3 around the phosphorylation site of Ser396. AA range:362-411
• Alternative Names    IRF3; Interferon regulatory factor 3; IRF-3
• Accession No. Swiss Prot:Q14653
  GeneID:3661
• UniprotQ14653
• Gene ID3661
• SDS-PAGE MW 60
• Concentration 1 mg/ml
• Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
• Storage -20˚C/1

• Western Blot: 1/500 - 1/2000.
  ELISA: 1/20000. Not yet tested in other applications.

interferon regulatory factor 3(IRF3) Homo sapiens This gene encodes a member of the interferon regulatory transcription factor (IRF) family. The encoded protein is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011],

• Western blot analysis of lysates from Jurkat cells treated with EGF 200ng/ml 30', using IRF-3 (Phospho-Ser396) Antibody. The lane on the right is blocked with the phospho peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting