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p44/42 MAP Kinase(Phospho-Tyr204) Antibody

11246
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  Catalog No. Package Size Price
11246-1 50ul $189.00
11246-2 100ul $279.00

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Description
  • Product Name p44/42 MAP Kinase(Phospho-Tyr204) Antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
  • Applications WB IHC IF
  • Species Reactivity Hu Ms Rt
  • Specificity The antibody detects endogenous level of p44/42 MAP Kinase only when phosphorylated at tyrosine 204.
  • Immunogen Type Peptide-KLH
  • Immunogen Description Peptide sequence around phosphorylation site of tyrosine 204 (T-E-Y(p)-V-A) derived from Human p44/42 MAP Kinase.
  • Target Name p44/42 MAP Kinase
  • Modification Phospho
  • Alternative Names ERK1, ERT2, ERK-1, PRKM3, P44ERK1
  • Accession No. Swiss-Prot: P27361
    NCBI Protein: NP_001035145.1
  • Concentration 1.0mg/ml
  • Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details

Predicted MW: 42 44 kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Images
  • p44/42 MAP Kinase(Phospho-Tyr204) Antibody - SAB | Signalway Antibody

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p44/42 MAP Kinase (Phospho-Tyr204) Antibody #11246 (left) or the same antibody preincubated with blocking peptide #51246 (right).

  • p44/42 MAP Kinase(Phospho-Tyr204) Antibody - SAB | Signalway Antibody

    Western blot analysis of extracts from SK-BR-3 cells, treated with insulin and EGF, and pretreated with U0126 and LY294002 cells using p44/42 MAP Kinase (Phospho-Tyr204) Antibody #11246.

  • p44/42 MAP Kinase(Phospho-Tyr204) Antibody - SAB | Signalway Antibody

    Immunofluorescence staining of methanol-fixed Hela cells showing centrosome and nuclear staining using p44/42 MAP Kinase (Phospho-Tyr204) Antibody #11246.

Background

Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1

TETE HANNKEN, et al. (2000) Am Soc Nephrol 11:1387-1397
Omar D. PerezNature et al. (2002) Biotechnology 20: 155 - 162
Jingui Yu, et al. (2005) Anesth Analg 101: 315-321
Hironobu Ihn et al.(2000) Immunology 165: 2149-2155
 

  • Jin-Hang Gao, Shi-Lei Wen, Wen-Juan Yang et al,Celecoxib Ameliorates Portal Hypertension of the Cirrhotic Rats through the Dual Inhibitory Effects on the Intrahepatic Fibrosis and Angiogenesis., PLoS ONE, 8(7): e69309 (2013),
    PMID: 23922700
  • Please let us know if you have published research using #11246 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
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