• Product NameDYKDDDDK-Tag Mouse Monoclonal Antibody
• Host SpeciesMouse
• ClonalityMonoclonal
• PurificationAffinity-chromatography
• ApplicationsWB IF/ICC IP
• Immunogen TypePeptide-KLH
• Immunogen DescriptionA synthetic peptide DYKDDDDK coupled to KLH.
• Target NameDYKDDDDK-Tag
• SDS-PAGE MW N/A
• Formulation Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20 °C.Stable for 12 months from date of receipt

• WB: 1:3000~1:10000

  IF/ICC: 1:200

 DYKDDDDK-Tag (FLAG tag） Mouse mAb is part of the series of Tag antibodies, the excellent quality in the research. FLAG tag antibody is a highly sensitive and affinity PAB applicable to FLAG-tagged fusion protein detection. FLAG tag antibody can detect FLAG tags in internal, C-terminal, or N-terminal recombinant proteins.

• DYKDDDDK-Tag (FLAG tag）Mouse Monoclonal antibody A: HEK 293 cell lysate without transfected B: HEK 293 cell lysate transfected with FLAG-tag protein C: IP (PBS + anti- FLAG mAb 4℃ overnight) D: IP (HEK293 transfected+protein G+anti- FLAG mAb 4℃ overnight) E: IP (HEK293 transfected+RIPA+Protein G 4℃ overnight) F: Multi Tag Recombinant protein (expressed in E.coli) TP=Target Protein HC=Heavy chain LC=Light Chain

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting