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Recombinant Human Interleukin-1 a (rHu IL-1a) #701011

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Catalog No. Package Size Price Quantity
701011 10ug $235.00
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Description
  • Product NameRecombinant Human Interleukin-1 a (rHu IL-1a)
  • Brief DescriptionRecombinant Protein
  • Host SpeciesE.coli
  • Purification> 97 % by SDS-PAGE and HPLC analyses.
  • Species ReactivityHu
  • Biological ActivityFully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 1.0 pg ml, corresponding to a specific activity of > 1.0 ⅹ 109 IU mg.
  • EndotoxinLess than 1.0 EU/μg of rHuIL-1a as determined by LAL method.
  • Target NamerHu IL-1a
  • Alternative Names    Lymphocyte-activating factor, LAF, Leukocyte Endogenous Mediator, LEM, Endogenous Pyrogen, EP, Mononuclear Cell Factor, MCF , Hematopoietin-1
  • Accession No. accession:P01583
    GeneID:3552
  • UniprotP01583
  • Gene ID3552;
  • Calculated MW Approximately 18.0 kDa, a sing
  • SDS-PAGE MW Sterile Filtered White lyophil
  • Target Sequence SAPFSFLSNV KYNFMRIIKY EFILNDALNQ SIIRANDQYL TAAALHNLDE AVKFDMGAYK SSKDDAKITV ILRISKTQLY VTAQDEDQPV LLKEMPEIPK TITGSETNLL FFWETHGTKN YFTSVAHPNL FIATKQDYWV CLAGGPPSIT DFQILENQA
  • Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
  • Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg mL. Stock solutions should be apportio
  • Storage This lyophilized preparation is stable at 2-8 ˚C, but should be kept at -20 ˚C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 ˚C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 ˚C to -70 ˚C. Avoid repeated freeze thaw cycles.
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Application

WBWestern Blotting

IHCImmunohistochemistry

IFImmunofluorescence

ICCImmunocytochemistry

FCFlow Cytometry

IPImmunoprecipitation

EELISA

DBDot Blotting

ChIPChromatin Immunoprecipitation

GICAGold Immunochromatography Assay

NCNegative Control

Species Reactivity

HuHuman

MsMouse 

RtRat 

Dm Drosophila melanogaster

C Caenorhabditis elegans

MkMonkey

RbRabbit

B Bovine 

D Dog

PPig

HmHamster

ChHm Chinese Hamster 

ChkChicken  

ShpSheep