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Recombinant Human Interleukin-3(rHu IL-3)

70103
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70103 10ug $235.00

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Description
  • Product Name Recombinant Human Interleukin-3(rHu IL-3)
  • Brief Description Recombinant Protein
  • Host Species E.coli
  • Purification > 96 % by SDS-PAGE and HPLC analyses.
  • Species Reactivity Hu
  • Biological Activity Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.1 ng ml, corresponding to a specific activity of > 1.0 ⅹ 107 IU mg.
  • Endotoxin Less than 1 EU/μg of rHuIL-3 as determined by LAL method.
  • Target Name rHu IL-3
  • Alternative Names Hematopoietic growth factor, MCGF, Multipotential colony-stimulating factor, P-cell-stimulating factor.
  • Accession No. accession:P08700
    GeneID:3562
  • Calculated MW Approximately 15.0 kDa, a sing
  • SDS-PAGE MW Sterile Filtered White lyophil
  • Target Sequence APMTQTTSLK TSWVNCSNMI DEIITHLKQP PLPLLDFNNL NGEDQDILME NNLRRPNLEA FNRAVKSLQN ASAIESILKN LLPCLPLATA APTRHPIHIK DGDWNEFRRK LTFYLKTLEN AQAQQTTLSL AIF
  • Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
  • Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg mL. Stock solutions should be apportio
  • Storage This lyophilized preparation is stable at 2-8 ˚C, but should be kept at -20 ˚C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 ˚C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 ˚C to -70 ˚C. Avoid repeated freeze thaw cycles.
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  • Recombinant Human Interleukin-3(rHu IL-3) - SAB | Signalway Antibody


Background
Interleukin-3 (IL-3) is an interleukin, a type of biological signal (cytokine) which is encoded by the IL-3 gene located on chromosome 5 and produced primarily by activated T cells beside human thymic epithelial cells, activated murine mast cells, murine keratinocytes and neurons astrocytes. The protein acts in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. The human IL-3 reported to be a monomer, as it is known, contains 133 amino acids residues which is a single non-glycosylated polypeptide. Specifically, human and murine IL-3 share low homology and it does not show activity on murine cells.
Regerences
1. Yang YC, Ciarletta AB, Temple PA, et al. 1986. Cell. 47:3-10. 2. Otsuka T, Miyajima A, Brown N, et al. 1988. J Immunol. 140:2288-95. 3. Dorssers L, Burger H, Bot F, et al. 1987. Gene. 55:115-24. 4. Feng Y, Klein BK, McWherter CA. 1996. J Mol Biol. 259:524-41.
    Please let us know if you have published research using #70103 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
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