- Product NameRecombinant Human Interleukin-12 (rHu IL-12)
- Brief DescriptionRecombinant Protein
- Host SpeciesCHO
- Purification> 95 % by SDS-PAGE and HPLC analyses.
- Species ReactivityHu
- Biological ActivityFully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using PHA-activated human T lymphoblasts is less than 0.05 ng ml, corresponding to a specific activity of > 2.0 ⅹ 107 IU mg.
- EndotoxinLess than 1 EU/μg of rHuIL-12 as determined by LAL method.
- Target NamerHu IL-12
- Alternative Names Natural killer cell stimulatory factor chain 2, NKSF2, Cytotoxic lymphocyte maturation factor 40 kDa subunit, CLMF p40
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Accession No. accession:P29459
GeneID:3592 - UniprotP29459
- Gene ID3592;
- Calculated MW Approximately 75 kDa, consisti
- SDS-PAGE MW Sterile Filtered White lyophil
- Target Sequence p35Subunit :RNLPVATPD PGMFPCLHHS QNLLRAVSNM LQKARQTLEF YPCTSEEIDH EDITKDKTST VEACLPLELT KNESCLNSRE TSFITNGSCL ASRKTSFMMA LCLSSIYEDL KMYQVEFKTM NAKLLMDPKR QIFLDQNMLA VIDELMQALN FNSETVPQKS SLEEPDFYKT KIKLCILLHA FRIRAVTIDR VMSYLNASp4 0Subunit:I WELKKDVYVV ELDWYPDAPG EMVVLTCDTP EEDGITWTLD QSSEVLGSGK TLTIQVKEFG DAGQYTCHKG GEVLSHSLLL LHKKEDGIWS TDILKDQKEP KNKTFLRCEA KNYSGRFTCW WLTTISTDLT FSVKSSRGSS DPQGVTCGAA TLSAERVRGD NKEYEYSVEC QEDSACPAAE ESLPIEVMVD AVHKLKYENY TSSFFIRDII KPDPPKNLQL KPLKNSRQVE VSWEYPDTWS TPHSYFSLTF CVQVQGKSKR EKKDRVFTDK TSATVICRKN ASISVRAQDR YYSSSWSEWA SVPCS
- Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
- Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg mL. Stock solutions should be apportio
- Storage This lyophilized preparation is stable at 2-8 ˚C, but should be kept at -20 ˚C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 ˚C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 ˚C to -70 ˚C. Avoid repeated freeze thaw cycles.
