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Recombinant Human EPO-a (rHu EPO-a)

70204
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70204 2000IU $235.00

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Description
  • Product Name Recombinant Human EPO-a (rHu EPO-a)
  • Brief Description Recombinant Protein
  • Purification > 98 % by SDS-PAGE and HPLC analyses.
  • Species Reactivity Hu
  • Biological Activity Fully biologically active when compared to standard. The Specific Activity was measured by the stimulation of reticulocyte production in normocyth-aemic mice and is no less than 1.5 ⅹ 105 IU mg.
  • Endotoxin Less than 0.01 EU/��g of rHuEPO-�� as determined by LAL method.
  • Target Name rHu EPO-a
  • Alternative Names Epoetin
  • Accession No. accession:P01588
    GeneID:2056
  • Calculated MW Mature human EPO, containing 1
  • SDS-PAGE MW Sterile Filtered White lyophil
  • Target Sequence APPRLICDSR VLERYLLEAK EAENITTGCA EHCSLNENIT VPDTKVNFYA WKRMEVGQQA VEVWQGLALL SEAVLRGQAL LVNSSQPWEP LQLHVDKAVS GLRSLTTLLR ALGAQKEAIS PPDAASAAPL RTITADTFRK LFRVYSNFLR GKLKLYTGEA CRTGDR
  • Formulation Lyophilized from a 0.2 μm filtered concentrated solution in sodium citrate buffer (1 liter of ddH2O containing 5.9 g of sodium citrate, 5.8 g of sodium chloride and 0.06 g of citric acid).
  • Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg mL. Stock solutions should be apportio
  • Storage This lyophilized preparation is stable at 2-8 ˚C, but should be kept at -20 ˚C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 ˚C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 ˚C to -70 ˚C. Avoid repeated freeze thaw cycles.
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  • Recombinant Human EPO-a (rHu EPO-a) - SAB | Signalway Antibody


Background

Erythropoietin (EPO), a glycoprotein produced primarily by the kidney, is the principal factor that regulates erythropoiesis by stimulating the proliferation and differentiation of erythroid progenitor cells. The production of EPO by kidney cells is increased in response to hypoxia or anemia. Recombinant EPO has been approved for the treatment of anemia associated with chronic renal failure as well as for anemia of AZT treated AIDS patients.

Regerences

1. Hanicki Z. 1988. Pol Arch Med Wewn, 80: 290-3. 2. Haniu M, Narhi LO, Arakawa T, et al. 1993. Protein Sci, 2: 1441-51. 3. Baranowska-Daca EandKsiazek A. 1994. Ann Univ Mariae Curie Sklodowska Med, 48 Suppl 3: 51-60. 4. Chow KM, Szeto CC, Li PK. 2003. Am J Kidney Dis, 41: 266-7; author reply 7. 5. Maurer MH, Schabitz WR, Schneider A. 2008. Curr Med Chem, 15: 1407-11.

    Please let us know if you have published research using #70204 so that we can cite your reference.
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    Protocol
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      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
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    • C Caenorhabditis elegans
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