• Product NameRecombinant Human SDF-1 a (rHu SDF-1 a/CXCL12- a)
• Brief DescriptionRecombinant Protein
• Host SpeciesE.coli
• Purification> 97 % by SDS-PAGE and HPLC analyses.
• Species ReactivityHu
• Biological ActivityFully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using PHA and rHuIL-2 activated human peripheral blood T-lymphocytes is in a concentration range of 20-80 ng ml.
• EndotoxinLess than 1 EU/ag of rHuSDF-1a/CXCL12a as determined by LAL method.
• Target NamerHu SDF-1a CXCL12-a
• Alternative Names    SDF-1 alpha, hSDF-1 alpha, IRH, hIRH, PBSF
• Accession No. accession:P48061
  GeneID:6387
• UniprotP48061
• Gene ID6387;
• Calculated MW Approximately 8.0 kDa, a singl
• SDS-PAGE MW Sterile Filtered White lyophil
• Target Sequence KPVSLSYRCP CRFFESHVAR ANVKHLKILN TPNCALQIVA RLKNNNRQVC IDPKLKWIQE YLEKALNK
• Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB pH 7.0, 130 mM NaCl.
• Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg mL. Stock solutions should be apportio
• Storage This lyophilized preparation is stable at 2-8 ˚C, but should be kept at -20 ˚C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 ˚C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 ˚C to -70 ˚C. Avoid repeated freeze thaw cycles.

CXCL12 also known as SDF-1 is belonging to the CXC chemokine family. It is encoded by the CXCL12 gene. In recently study, Human CXCL12 is expressed as six isoforms that differ only in the C-terminal tail. And all SDF-1 isoforms undergo proteolytic processing of the first two N-terminal amino acids. Contrast to the canonical sequence SDF-1β, SDF-1α is shorter by four amino acids at the C-terminal tail. On the cell surface, the receptor for this chemokine is CXCR4 and syndecan4. CXCL12 is strongly chemotactic for T-lymphocytes, monocytes, but not neutrophils. CXCL12 is a very important factor in carcinogenesis and the neovascularisation linked to tumor progression.

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• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting