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Goat anti-Mouse IgG Secondary Antibody HRP conjugated

L3032-2
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  Catalog No. Package Size Price
L3032-2 1ml $85.00

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Description
  • Product Name Goat anti-Mouse IgG Secondary Antibody HRP conjugated
  • Brief Description Secondary Antibody
  • Clonality Polyclonal
  • Purification The antibody was obtained by affinity purified technique, which removes essentially all goat serum proteins, except the specific antibody for mouse IgG. Goat anti-mouse IgG was conjugated to peroxidase by means of a method described by Wilson MB and Nakane PK.
  • Applications ELISA WB IHC
  • Species Reactivity Ms
  • Specificity The antibody conjugate is specific for mouse IgG. No cross-reactivity was observed with mouse IgA, IgM proteins by ELISA.
  • Immunogen Description The antiserum was developed in goat using the mouse IgG as the immunogen.
  • Conjugates HRP
  • Target Name Mouse IgG
  • Formulation HRP-labeled Goat IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol.
  • Storage Store at -20 ℃/1 year
Application Details

ELISA : 1:5000~1:20000
WB : 1:5000~1:10000
IHC 1:1000~5000

Regerences
Wilson MB and Nakane PK. In Immunofluorescence and Related 
Staining Techniques, Elsevier/North Holland Biomedical Press, 
Amsterdam, P215 (1978).
 
  • Jossimara Polettini, Eliane Passarelli Vieira, Mariana Perlati dos Santos et al,Interleukin 18 messenger RNA and proIL-18 protein expression in chorioamniotic membranes from pregnant women with preterm prelabor rupture of membranes, European Journal of Obstetrics & Gynecology andReproductive Biology, 161 (2012) 134¨C139 (2012),
    PMID: 22285684
  • Lei Wu, Ya-nan Jiang, Qian Tang et al,Development of an Aeromonas hydrophila recombinant extracellular protease vaccine., Microbial Pathogenesis., 53:183-188 (2012),
    PMID: 22874879
  • Please let us know if you have published research using #L3032-2 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  
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