• Product NameMembrane Protein Extraction Kit

1.Storage the Protease Inhibitor Cocktail at -20?
2.Storage the extraction solution at 2-8?

The Membrane Protein Extraction Reagent Kit is for the enrichment of integral membrane proteins from cultured mammalian cells or from mammalian tissue using a mild detergent -based protocol. The cells are first lysed with a detergent, after which a second detergent is added to solubilize the membrane proteins. The cocktail is incubated at 37°C to separate the hydrophobic proteins from the hydrophilic proteins through phase partitioning.
Extraction efficiencies will vary depending on the number of times the integral membrane proteins of interest spans the lipid bilayer. Membrane proteins with up to five transmembrane domains are typically extracted with an efficiency of up to 90%. Cross -contamination of cytosolic proteins into the membrane fraction is typically < 10%.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting