• Product NameFKHRL1(Phospho-Ser253) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB IHC IF
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of FKHRL1 only when phosphorylated at serine 253.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of serine 253(A-V-S(p)-M-D) derived from Human FKHRL1.
• Target NameFKHRL1
• ModificationPhospho
• Alternative Names    FOXO2; AF6q21; FKHRL1; FOXO3A; FKHRL1P2
• Accession No. Swiss-Prot: O43524
  NCBI Gene ID: 2309
  NCBI mRNA: NM_001455.3
  NCBI Protein: NP_001446.1
• UniprotO43524
• Gene ID2309;
• Calculated MW 90kD
• Concentration 1.0mg/ml
• Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
• Storage Store at -20°C

• Predicted MW: 97kd

  Western blotting: 1:500~1:1000

  Immunohistochemistry: 1:50~1:100

  Immunofluorescence: 1:100~1:200

This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008],

• Western blot analysis of extracts from NIH/3T3 cells using FKHRL1 (Ab-253) antibody (#21171, Lane 1 and 2) and FKHRL1 (phospho-Ser253) antibody (#11157, Lane 3 and 4).
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using FKHRL1 (phospho-Ser253) antibody (#11157).
• Immunofluorescence staining of methanol-fixed HeLa cells using FKHRL1 (phospho-Ser253) antibody (#11157, Red).
• Western Blot analysis of HELA cells using Phospho-FoxO3A (S253) Polyclonal Antibody diluted at 1:2000

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting