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Histone H4R3me2a Polyclonal Antibody

HW017
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  Catalog No. Package Size Price
HW017 100ul $299.00

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Description
  • Product Name Histone H4R3me2a Polyclonal Antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Purification Antibodies were produced by immunizing rabbits and were purified by antigen affinity-chromatography.
  • Applications WB IF IP ChIP
  • Species Reactivity Hu Ms Rt
  • Immunogen Type Peptide
  • Immunogen Description A synthetic methylated peptide corresponding to residues surrounding Arg3 of human histone H4
  • Target Name Histone H4
  • Modification Methyl
  • Alternative Names H3t; H3.4; H3/g; H3FT;
  • Accession No. Gene ID: 8370 Swiss Prot: P62805
  • SDS-PAGE MW 11kDa
  • Concentration 1.0mg/ml
  • Formulation Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage Store at -20℃ or -80℃. Avoid freeze / thaw cycles.
Application Details

WB 1:500 - 1:2000

IF 1:50- 1:200
IP 1:50- 1:100

ChIP 1:50- 1:200

Images
  • Histone H4R3me2a Polyclonal Antibody - SAB | Signalway Antibody

    Western blot analysis of extracts of HeLa cell line and H3 protein expressed in E.coli., using H4R3 me2a antibody.

  • Histone H4R3me2a Polyclonal Antibody - SAB | Signalway Antibody

    Dot-blot analysis of all sorts of methylation peptidesusing H4R3 me2a antibody.

  • Histone H4R3me2a Polyclonal Antibody - SAB | Signalway Antibody

    Immunofluorescence analysis of 293T cell using H4R3me2a antibody. Blue: DAPI for nuclear staining.

Background

 Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element.

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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  
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