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PKM2 Antibody

JM001
Review: comment rank 5 pdf
  Catalog No. Package Size Price
JM001-1 50ul $189.00
JM001-2 100ul $279.00

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Description
  • Product Name PKM2 Antibody
  • Host Species Mouse
  • Clonality Monoclonal
  • Clone No. XD2014M001S1
  • Isotype IgG
  • Applications WB IP
  • Species Reactivity Hu
  • Specificity The antibody detects endogenous levels of total PKM2 protein
  • Immunogen Type Recombinant Protein
  • Immunogen Description Recombinant human PKM2 protein
  • Target Name PKM2
  • Alternative Names PKM; PK3; OIP3; PK2;
  • Accession No. Swiss-Prot: P14618-1 NCBI Protein: NP_872270.1
  • SDS-PAGE MW 60kd
  • Concentration 1.0mg/ml
  • Formulation 1.0 mg/mL in phosphate buffer without Mg2+/Ca2+, pH7.4, 150mM NaCl and 50% glycerol
  • Storage Store at -20°C
Application Details

Western Blotting: 1:500
IP: 1 ul/500ug cell lysate

Images
  • PKM2 Antibody - SAB | Signalway Antibody

    Western blot analysis of extracts from U87 cells using PKM2 Antibody #JM001.

  • PKM2 Antibody - SAB | Signalway Antibody

    Immunoprecipitation analysis of U87 cell lysates using PKM2 antibody. IP:PKM2 Monoclonal Antibody #JM001. WB:PKM2 Polyclonal Antibody #JM001.

Background

 Pyruvate kinase (PK) regulates the final rate-limiting step of glycolysis in the production of pyruvate and adenosine triphosphate (ATP). Alternate splicing of PKM pre-mRNA leads to PKM2 generation by the inclusion of exon 10 and the exclusion of exon 9, which is specific for PKM1. Besides its cytosolic roles in glycolysis, PKM2, which is upregulated by growth factor receptor activation 1, is phosphorylated at S37 by extracellular signal-regulated kinase (ERK) 2. This phosphorylation leads to the cis-trans isomerization of PKM2 by the peptidyl-prolineisomerase protein interacting with never in mitosis A 1 (PIN1), exposure of the nuclear localization signal (NLS) of PKM2, and subsequent binding of importin α 5 for nuclear translocation 2. In the nucleus, PKM2binds to phosphorylated Y333 of β-catenin, which is essential for β-catenin transactivation 3, and interacts with and phosphorylates histone H3 at T11, leading to H3-K9 acetylation and transcription of genes such as MYC and CCND14. c-Myc expression results in the upregulation of GLUT1, lactate dehydrogenase A (LDHA), and, in a positive feedback loop, PTB-dependent PKM2, which subsequently enhances the Warburg effect 2. Cyclin D1 expression, in turn, promotes G1-S phase transition 3, 4.

1 Yang W, Xia Y, Cao Y et al. EGFR-induced and PKCepsilonmonoubiquitylation-dependent NF-kappaB activation upregulates PKM2 expression and promotes tumorigenesis. Molecular cell 2012; 48:771-784.
2 Yang W, Zheng Y, Xia Y et al. ERK1/2-dependent phosphorylation and nuclear translocation of PKM2 promotes the Warburg effect. Nature cell biology 2012; 14:1295-1304.
3 Yang W, Xia Y, Ji H et al. Nuclear PKM2 regulates beta-catenin transactivation upon EGFR activation. Nature 2011; 480:118-122.
4 Yang W, Xia Y, Hawke D et al. PKM2 phosphorylates histone H3 and promotes gene transcription and tumorigenesis. Cell 2012; 150:685-696.
 
    Please let us know if you have published research using #JM001 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  
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