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CDK1/CDC2 (Phospho-Thr14) Antibody

11997
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  Catalog No. Package Size Price
11997-1 50ul $189.00
11997-2 100ul $279.00

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Description
  • Product Name CDK1/CDC2 (Phospho-Thr14) Antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
  • Applications WB
  • Species Reactivity Hu Ms Rt
  • Specificity The antibody detects endogenous level of CDK1/CDC2 only when phosphorylated at threonine 14.
  • Immunogen Type Peptide-KLH
  • Immunogen Description Peptide sequence around phospho rylation site of threonine14(E-G-T(p)-Y-G) derived from Human CDK1/CDC2 .
  • Target Name CDK1/CDC2
  • Modification Phospho
  • Alternative Names CDC28; CDC2A; CDK1; MPF; kinase Cdc2
  • Accession No. Swiss-Prot#: P06493
    NCBI Gene#: 983
    NCBI mRNA#:NM_001170406.1
    NCBI Protein#:NP_001163877.1
  • SDS-PAGE MW 34kd
  • Concentration 1.0mg/ml
  • Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage Store at -20˚C/1 year
Application Details

Western blotting: 1:500~1:1000

Images
  • CDK1/CDC2 (Phospho-Thr14) Antibody - SAB | Signalway Antibody

    Western blot analysis of extracts from HepG2 cells treated with Forskolin using Phospho-CDK1/CDC2 (Thr14) antibody #11997.The lane on the right is treated with the antigen-specific peptide.

Background

Serine/threonine-protein kinase involved in the control of the cell cycle; essential for meiosis, but dispensable for mitosis. Phosphorylates CTNNB1, USP37, p53/TP53, NPM1, CDK7, RB1, BRCA2, MYC, NPAT, EZH2. Interacts with cyclins A, B1, B3, D, or E. Triggers duplication of centrosomes and DNA. Acts at the G1-S transition to promote the E2F transcriptional program and the initiation of DNA synthesis, and modulates G2 progression; controls the timing of entry into mitosis/meiosis by controlling the subsequent activation of cyclin B/CDK1 by phosphorylation, and coordinates the activation of cyclin B/CDK1 at the centrosome and in the nucleus.

Sarkar S, Dey BK, Dutta A (2010) Mol Biol Cell 21, 2138-49
Shreeram S, Hee WK, Bulavin DV (2008) Mol Cell Biol 28, 7442-50
Mayya V, et al. (2006) Mol Cell Proteomics 5, 1146-57

    Please let us know if you have published research using #11997 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  
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