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CaMK2α/β/δ (Phospho-Thr305) Antibody

11644
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  Catalog No. Package Size Price
11644-1 50ul $189.00
11644-2 100ul $279.00

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Description
  • Product Name CaMK2α/β/δ (Phospho-Thr305) Antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
  • Applications WB IHC
  • Species Reactivity Hu Ms
  • Specificity The antibody detects endogenous levels of CaMKII only when phosphorylated at threonine 305.
  • Immunogen Type Peptide-KLH
  • Immunogen Description Peptide sequence around phosphorylation site of threonine 305 (I-L-T(p)-T-M) derived from Human CaMK2α/β/δ.
  • Target Name CaMK2α/β/δ
  • Modification Phospho
  • Alternative Names CAMK2A; KCC2A; kinase CaMK2-alpha; CaMKII-alpha;
  • Accession No. Swiss-Prot#: Q9UQM7;
    NCBI Gene#: 816/817;
    NCBI Protein#: NP_741960.1.
  • SDS-PAGE MW 54kd
  • Concentration 1.0mg/ml
  • Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage Store at -20˚C/1 year
Application Details

Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Images
  • CaMK2α/β/δ (Phospho-Thr305) Antibody - SAB | Signalway Antibody

    Western blot analysis of extracts from 3T3 cells (Lane 2), using CaMK2α/β/δ (Phospho-Thr305) Antibody #11644. The lane on the left is treated with antigen-specific peptide.

  • CaMK2α/β/δ (Phospho-Thr305) Antibody - SAB | Signalway Antibody

    Immunohistochemical analysis of paraffin-embedded human brain tissue using CaMKII (Phospho-Thr305) antibody #11644 (left)or the same antibody preincubated with blocking peptide (right).

Background

Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca2+/calmodulin-binding and autophosphorylation, and is involved in dendritic spine and synapse formation, neuronal plasticity and regulation of sarcoplasmic reticulum Ca2+ transport in skeletal muscle. In neurons, plays an essential structural role in the reorganization of the actin cytoskeleton during plasticity by binding and bundling actin filaments in a kinase-independent manner. This structural function is required for correct targeting of CaMK2A, which acts downstream of NMDAR to promote dendritic spine and synapse formation and maintain synaptic plasticity which enables long-term potentiation (LTP) and hippocampus-dependent learning. In developing hippocampal neurons, promotes arborization of the dendritic tree and in mature neurons, promotes dendritic remodeling. Participates in the modulation of skeletal muscle function in response to exercise. In slow-twitch muscles, is involved in regulation of sarcoplasmic reticulum (SR) Ca2+ transport and in fast-twitch muscle participates in the control of Ca2+ release from the SR through phosphorylation of triadin, a ryanodine receptor-coupling factor, and phospholamban (PLN/PLB), an endogenous inhibitor of SERCA2A/ATP2A2.

Carl W. Tong, J. Physiol., Aug 2004; 558: 927 - 941.
Pierre R, J. Biol. Chem., Sep 1997; 272: 24133.
Daliang Wang, PNAS, Jun 1998; 95: 7133.

    Please let us know if you have published research using #11644 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  
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