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GSK3β(Phospho-Ser9) Antibody

11002
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  Catalog No. Package Size Price
11002-1 50ul $189.00
11002-2 100ul $279.00

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Description
  • Product Name GSK3β(Phospho-Ser9) Antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
  • Applications WB IHC IF
  • Species Reactivity Hu Ms Rt
  • Specificity The antibody detects endogenous level of GSK3 beta only when phosphorylated at serine 9.
  • Immunogen Type Peptide-KLH
  • Immunogen Description Peptide sequence around phosphorylation site of serine 9 (T-T-S(p)-F-A) derived from Human GSK3β.
  • Target Name GSK3β
  • Modification Phospho
  • Alternative Names Factor A, GSK-3 beta, Protein kinase GSK-3-beta, kinase GSK-3 beta
  • Accession No. Swiss-Prot: P49841
    NCBI Protein: NP_001139628.1
  • SDS-PAGE MW 46kd
  • Concentration 1.0mg/ml
  • Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage Store at -20°C for long term preservation (recommended). Avoid Store at 4°C for short term use.
Application Details

Predicted MW: 46kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Images
  • GSK3β(Phospho-Ser9) Antibody - SAB | Signalway Antibody

    Western blot analysis of extracts from 293 cells untreated(lane 1) or treated with serum(lane 2) using GSK3β(Phospho-Ser9) Antibody #11002.

  • GSK3β(Phospho-Ser9) Antibody - SAB | Signalway Antibody

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using GSK3β (Phospho-Ser9) Antibody #11002 (left) or the same antibody preincubated with blocking peptide #51002 (right).

  • GSK3β(Phospho-Ser9) Antibody - SAB | Signalway Antibody

    Immunofluorescence staining of methanol-fixed Hela cells showing cytoplasmic staining using GSK3β(Phospho-Ser9) Antibody #11002.

  • GSK3β(Phospho-Ser9) Antibody - SAB | Signalway Antibody

    Immunohistochemical analysis of paraffin-embedded human Lung carcinoma tissue using GSK3β (Phospho-Ser9) Antibody #11002.

  • GSK3β(Phospho-Ser9) Antibody - SAB | Signalway Antibody

    Immunofluorescence staining of methanol-fixed Hela cells showing cytoplasmic staining using GSK3β (Phospho-Ser9) Antibody #11002.

  • GSK3β(Phospho-Ser9) Antibody - SAB | Signalway Antibody

    Western blot analysis of extracts from Hela cells, treated with EGF or calf intestinal phosphatase (CIP), using GSK3β (Phospho-Ser9) Antibody #11002.

Background

Participates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates MUC1 in breast cancer cells, and decreases the interaction of MUC1 with CTNNB1/beta-catenin. Phosphorylates CTNNB1/beta-catenin.

Fan G, et al. (2003) J Biol Chem. 278(52): 52432-52436.
Barry FA, et al. (2003) FEBS Lett. 553(1-2): 173-178.
Welsh, et al. (1996) Trends Cell Biol. 6: 274-279.
Srivastava A K, et al. (1998) Mol Cell Biochem. 182: 135-141.

  • Cong REN, Jia-Mou LI, Xin LIN et al,LIPUS Enhance Elongation of Neurites in Rat Cortical Neurons through Inhibition of GSK-3¦Â., Biomedical and Environmental Sciences, 23(3):244-249 (2010),
    PMID: 20708505
  • Estefan¨ªa de Munck, Emma Mu?noz-S¨¢ez, Bego?na G. Miguel et al,B-N-methylamino-l-alanine causes neurological andpathological phenotypes mimicking Amyotrophic LateralSclerosis (ALS): The first step towards an experimentalmodel for sporadic ALS. , environmental toxicology and pharmacology, 36:243¨C255 (2013),
    PMID: 23688553
  • Wen-Fei Tan, Xue-Zhao Cao, Jun-KeWang et al,Protective effects of lithium treatment for spatial memory deficits induced by tau hyperphosphorylation in splenectomized rats., Clinical and Experimental Pharmacology and Physiology., 37(10):1010¨C1015 (2010),
    PMID: 20659131
  • Yingjuan Yang, Jinzeng Yang, Rongxin Liu et al,Accumulation of ¦Â-catenin by lithium chloride in porcine myoblast cultures accelerates cell differentiation., Molecular Biology Reports, Volume 38, Number 3, Pages 2043-2049 (2011),
    PMID: 20857211
  • Yu-fei Pan, Li-wei Dong, Min Wang et al,Signal regulatory protein ¦Á negatively regulates mast-cell activation following Fc¦ÅRI aggregation., Eur. J. Immunol., 43(6):1598¨C1607 (2013),
    PMID: 23504624
  • Please let us know if you have published research using #11002 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  
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