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Chk2 (Phospho-Thr383) Antibody

11683
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  Catalog No. Package Size Price
11683-1 50ul $189.00
11683-2 100ul $279.00

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Description
  • Product Name Chk2 (Phospho-Thr383) Antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
  • Applications WB IF
  • Species Reactivity Hu Ms Rt Mk
  • Specificity The antibody detects endogenous levels of Chk2 only when phosphorylated at threonine 383.
  • Immunogen Type Peptide-KLH
  • Immunogen Description Peptide sequence around phosphorylation site of threonine383 (M-R-T(p)-L-C) derived from Human Chk1.
  • Target Name Chk2
  • Modification Phospho
  • Alternative Names CHEK2; CHK2; Cds1; Chk2; RAD53
  • Accession No. Swiss-Prot#: O96017;
    NCBI Gene#: 11200;
    NCBI Protein#: NP_009125.1.
  • SDS-PAGE MW 60kd
  • Concentration 1.0mg/ml
  • Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage Store at -20˚C/1 year
Application Details

Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200

Images
  • Chk2 (Phospho-Thr383) Antibody - SAB | Signalway Antibody

    Western blot analysis of extracts from COS7 cells treated with UV using Chk2 (Phospho-Thr383) Antibody #11683.The lane on the right is treated with the antigen-specific peptide.

  • Chk2 (Phospho-Thr383) Antibody - SAB | Signalway Antibody

    Immunofluorescence staining of methanol-fixed HeLa cells using Chk2 (Phospho-Thr383) Antibody #11683.

Background

In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by Chk2 gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants encoding different isoforms have been found for this gene.

Shannon L. Gibson, Cancer Res., Dec 2005; 65: 10734 - 10741.
Jinwoo Ahn, J. Biol. Chem., Dec 2002; 277: 48418 - 48426.
Nabil H. Chehab, Genes & Dev., Feb 2000; 14: 278.

    Please let us know if you have published research using #11683 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  
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