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LDHA Antibody

32182
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  Catalog No. Package Size Price
32182 100ul $279.00

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Description
  • Product Name LDHA Antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Purification Antibodies were purified by affinity purification using immunogen.
  • Applications WB IHC IF
  • Species Reactivity Hu Ms Rt
  • Specificity The antibody detects endogenous level of total LDHA protein.
  • Immunogen Type Recombinant Protein
  • Immunogen Description Recombinant protein of human LDHA .
  • Target Name LDHA
  • Alternative Names LDHA; LDH-M; LDH1; PIG19;
  • Accession No. Swiss-Prot:P00338
    NCBI Gene ID:3939
  • SDS-PAGE MW 37KD
  • Concentration 1.0mg/ml
  • Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage Store at -20˚C
Application Details

Western blotting: 1:500 - 1:2000
Immunohistochemistry: 1:50 - 1:100
Immunofluorescence: 1:10 - 1:100

Images
  • LDHA Antibody - SAB | Signalway Antibody

    Western blot analysis of Jurkat cell and A431 cell lysate using LDHA antibody.

  • LDHA Antibody - SAB | Signalway Antibody

    Immunohistochemical analysis of paraffin-embedded rat lung using LDHA antibody at dilution of 1:200 (200x lens).

  • LDHA Antibody - SAB | Signalway Antibody

    Immunofluorescence analysis of A549 cell using LDHA antibody. Blue: DAPI for nuclear staining

Background

Lactate dehydrogenase (LDH) catalyzes the interconversion of pyruvate and NADH to lactate and NAD+. When the oxygen supply is too low for mitochondrial ATP production, this reaction recycles NADH generated in glycolysis to NAD+, which reenters glycolysis. The major form of LDH found in muscle cells is the A (LDHA) isozyme. The LDHA promoter contains HIF-1α binding sites (1). LDHA expression is induced under hypoxic conditions (2). During intensive and prolonged muscle exercise, lactate accumulates in muscle cells when the supply of oxygen does not meet demand. When oxygen levels return to normal, LDH converts lactate to pyruvate to generate ATP in the mitochondrial electron transport chain.

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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  
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