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Cytochrome C Mouse Monoclonal Antibody FITC Conjugated(6B10)

C10132F
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  Catalog No. Package Size Price
C10132F 100ul $409.00

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Description
  • Product Name Cytochrome C Mouse Monoclonal Antibody FITC Conjugated(6B10)
  • Host Species Mouse
  • Clonality Monoclonal
  • Clone No. 6B10
  • Isotype IgG
  • Purification Purified by Protein A.
  • Applications IF(IHC-P)
  • Species Reactivity Hu Rt
  • Immunogen Description Recombinant human Cytochrome C Protein
  • Conjugates FITC
  • Excitation Emission 494nm 518nm
  • Target Name Cytochrome C
  • Alternative Names Cytochrome c; CYCS
  • Accession No. Swiss-Prot#P99999
    NCBI Gene ID54205
  • Concentration 1mg ml
  • Formulation 10mM Tris Buffered Saline containing 1% BSA, 50% glycerol and 0.09% sodium azide.
  • Storage Store at 4C for 12 months.
Application Details

Immunofluorescence: 1:50-200

Background

Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain. Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.

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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  
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