• Product Name6-His Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB
• SpecificityThe antibody detects transfected proteins contanining 6-His tag.
• Immunogen TypePeptide
• Immunogen DescriptionSynthetic peptide: 18XHis conjugated to KLH
• Target Name6-His
• Alternative Names    6 His epitope tag
• SDS-PAGE MW 46kd
• Concentration 0.5mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500-1:2000
  

The 6 X histidine motif is used as a tag on many recombinant proteins to facilitate purification.It can then be purified using immobilized metal ions,traditionally nickel,in a procedure termed immobilized-metal affinity chromatography.Further analysis of the protein can be achieved using an antibody specific to the 6 X histidine in techniques such as western blotting.The antibody recognizes the His-tag fused to the carboxy- termini of targeted proteins in transfected or transformed cells.

• Gel: 8%SDS-PAGE
  Lysate: 2ug Recombinant 6-HIS Tagged protein
  Primary antibody: 1/500 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 5 seconds

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting