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ACTIN Antibody

35537
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  Catalog No. Package Size Price
35537 100ul $279.00

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Description
  • Product Name ACTIN Antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Purification Antigen affinity purification.
  • Applications WB
  • Species Reactivity Plant
  • Specificity The antibody detects endogenous levels of total ACTIN protein.
  • Immunogen Type Peptide
  • Immunogen Description Synthetic peptide corresponding to residues near the N terminal of Arabidopsis thaliana actin
  • Target Name ACTIN
  • Alternative Names ACTB
  • Accession No. Swiss-Prot#: P53497
    NCBI Gene ID: 823805
    Gene Accssion: NP_190236.1
  • SDS-PAGE MW 42kd
  • Concentration 1mg/ml
  • Formulation Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol.
  • Storage Store at -20˚C
Application Details

Western blotting: 1:500-1:2000

Images
  • ACTIN Antibody - SAB | Signalway Antibody

    Gel: 10%SDS-PAGE
    Lysates (from left to right): Cinnamomum camphora leaves, Nicotiana tabacum leaves, Triticum aestivuml leaves, Arabidopsis thaliana leaves, Brassica campestris flowers, Brassica campestris leaves, Vicit Faba leaves
    Amount of lysate: 50ug per lane
    Primary antibody: 1/500 dilution
    Secondary antibody dilution: 1/8000
    Exposure time: 20 seconds

Background

Member of actin subclass composed of ACT12 and ACT4. RNA is expressed at very low levels in vegetative organs, low levels in flowers and very high levels in pollen. Expression of an ACT12/GUS fusion was found in vascular tissues, tapetum, developing and mature pollen, the root cap and in a ring of pericycle tissues during lateral root initiation and early development.

    Please let us know if you have published research using #35537 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  
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