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TUBB3 Antibody

35539
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  Catalog No. Package Size Price
35539 100ul $279.00

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Description
  • Product Name TUBB3 Antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Purification Antigen affinity purification.
  • Applications WB IHC
  • Species Reactivity Hu Ms Rt
  • Specificity The antibody detects endogenous levels of total TUBB3 protein.
  • Immunogen Type Recombinant Protein
  • Immunogen Description Fusion protein corresponding to a region derived from internal residues of human Tubulin beta-3 chain
  • Target Name TUBB3
  • Alternative Names TUBB4
  • Accession No. Swiss-Prot#: Q13509
    NCBI Gene ID: 10381
    Gene Accssion: BC000748
  • SDS-PAGE MW 50kd
  • Concentration 0.5mg/ml
  • Formulation Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol.
  • Storage Store at -20˚C
Application Details

Western blotting: 1:500-1:1000
Immunohistochemistry: 1:25-1:50

Images
  • TUBB3 Antibody - SAB | Signalway Antibody

    Gel: 8%SDS-PAGE
    Lysate:30ug Human brain glioma tissue
    Primary antibody: 1/500 dilution
    Secondary antibody dilution: 1/8000
    Exposure time: 10 seconds

  • TUBB3 Antibody - SAB | Signalway Antibody

    Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissue using #35539 at dilution 1/25.

  • TUBB3 Antibody - SAB | Signalway Antibody

    Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue using #35539 at dilution 1/25.

Background
his gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6
    Please let us know if you have published research using #35539 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  
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