• Product NameEzrin(Phospho-Tyr353) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of Ezrin only when phosphorylated at tyrosine 353.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of tyrosine 353 (Q-D-Y(p)-E-E) derived from Human EZRIN.
• Target NameEzrin
• ModificationPhospho
• Alternative Names    Cytovillin; EZRI; VIL2; Villin 2; p81
• Accession No. Swiss-Prot: P15311
  NCBI Protein: NP_001104547.1
• UniprotP15311
• Gene ID7430;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 81kd
  Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100
   

Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis.

Zhao H, et al. (2004) Proc Natl Acad Sci U S A101 (25): 9485-9490.
Wang Q, et al. (2003) J Biol Chem 278(48): 47731-47743.
Gautreau A, et al. (1999) Proc Natl Acad Sci U S A 96(13): 7300-7305.
Crepaldi T, et al. (1997) J Cell Biol 138(2): 423-34.
 

• Western blot analysis of extracts from 293 cells untreated or treated with EGF using Ezrin(Phospho-Tyr353) Antibody #11063.
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Ezrin(Phospho-Tyr353) Antibody #11063(left) or the same antibody preincubated with blocking peptide(right).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting