• Product NameErbB-2/HER-2 Mouse Monoclonal Antibody
• Host SpeciesMouse
• ClonalityMonoclonal
• Clone No.11H9
• PurificationAffinity purification using immunogen.
• ApplicationsWB,IHC,IF
• Species ReactivityHu Ms Rt
• SpecificityThe ErbB-2/HER-2 Mouse Monoclonal antibody detects endogenous ErbB-2/HER-2 proteins.
• Target NameErbB-2/HER-2
• Alternative Names    c-erb B2/neu protein; CD340; erbB-2; ERBB2; HER-2; HER-2/neu; HER2; herstatin; Metastatic lymph node gene 19 protein; MLN 19;
• Accession No. Swiss-Prot#:P04626
• UniprotP04626
• Gene ID2064;
• SDS-PAGE MW 138kd
• Concentration 1.0mg/ml
• Formulation Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB dilution: 1:2000~1:4000
  IHC dilution: 1:200
  
  IF dilution:1:200

ErbB 2 is a receptor tyrosine kinase of the ErbB 2 family. It is closely related instructure to the epidermal growth factor receptor. ErbB 2 oncoprotein is detectable in a proportion of breast and other adenocarconomas, as well as transitional cell carcinomas. In the case of breast cancer, expression determined by immunohistochemistry has been shown to be associated with poor prognosis.

• Western blot analysis of Hela cell lysate (lane 1) and mouse brain (lane 2), using #37997 diluted at 1:4,000.
• IHC staining of human breast cancer tissue with ErbB-2/HER-2 mouse mAb(11H9) diluted at 1:200.
• Immunofluorescence analysis of Mouse-spleen tissue. 1,HER2 Monoclonal Antibody(11H9)(red) was diluted at 1:200(4C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
• Immunofluorescence analysis of Rat-spleen tissue. 1,HER2 Monoclonal Antibody(11H9)(red) was diluted at 1:200(4C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
• Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. 1,HER2 Monoclonal Antibody(11H9) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
• Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1,HER2 Monoclonal Antibody(11H9) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
• Immunohistochemical analysis of paraffin-embedded Mouse-testis tissue. 1,HER2 Monoclonal Antibody(11H9) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting