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PCNA Mouse Monoclonal Antibody

37978
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  Catalog No. Package Size Price
37978 100ul $279.00

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Description
  • Product Name PCNA Mouse Monoclonal Antibody
  • Host Species Mouse
  • Clonality Monoclonal
  • Purification Affinity purification using immunogen.
  • Applications WB,IHC,IF
  • Species Reactivity Hu Ms
  • Specificity Antibody can detect endogenous?PCNA protein.
  • Target Name PCNA
  • SDS-PAGE MW 36kd
  • Concentration 1.0mg/ml
  • Formulation Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage Store at -20˚C
Application Details

WB dilution: 1:5000
IHC dilution: 1:200

IF dilution:1:200

Images
  • PCNA Mouse Monoclonal Antibody - SAB | Signalway Antibody

    Western blot analysis of Hela (lane 1), Rat brain (lane 2), NIH 3T3 (lane 3), 293T (lane 4), using #37978 diluted at 1:5000.

  • PCNA Mouse Monoclonal Antibody - SAB | Signalway Antibody

    IHC staining of paraffin- embedded Human breast cancer tissue with PCNA mouse mAb (12D10) diluted at 1:200.

  • PCNA Mouse Monoclonal Antibody - SAB | Signalway Antibody

    Immunofluorescence analysis of Human-lung-cancer tissue. 1,PCNA Monoclonal Antibody(12D10)(red) was diluted at 1:200(4C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

  • PCNA Mouse Monoclonal Antibody - SAB | Signalway Antibody

    Immunofluorescence analysis of Rat-testis tissue. 1,PCNA Monoclonal Antibody(12D10)(red) was diluted at 1:200(4C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

  • PCNA Mouse Monoclonal Antibody - SAB | Signalway Antibody

    Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1,PCNA Monoclonal Antibody(12D10) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

  • PCNA Mouse Monoclonal Antibody - SAB | Signalway Antibody

    Immunohistochemical analysis of paraffin-embedded Rat-testis tissue. 1,PCNA Monoclonal Antibody(12D10) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

  • PCNA Mouse Monoclonal Antibody - SAB | Signalway Antibody

    Immunohistochemical analysis of paraffin-embedded Mouse-liver tissue. 1,PCNA Monoclonal Antibody(12D10) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

Background
PCNA is a marker for cells in early G1 phase and S phase of the cell cycle. It is found in the nucleus and is a cofactor of DNA polymerase delta. PCNA acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, PCNA is ubiquitinated and is involved in the RAD6 dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for PCNA. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.
    Please let us know if you have published research using #37978 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  
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