• Product NameHistone H2A(Phospho-Ser129) Rabbit Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB
• Species ReactivityHu Rt Ms
• SpecificityThe Histone H2A(Phospho Ser129) Rabbit Polyclonal Antibody detects endogenous Histone H2A (Phospho Ser129) protein.
• Immunogen Typepeptide
• Immunogen DescriptionPeptide sequence around phosphorylation site of serine 129(K-A-Q-S(p)-K) derived from Human Histone H2A.
• Target NameHistone H2A(Phospho-Ser129)
• ModificationPhospho
• Alternative Names    H2A 615 antibody; H2A GL101 antibody; H2A.1 antibody; HIST1H2AE antibody; MGC74460 antibody
• Accession No. Swiss-Prot#:Q96QV6
• UniprotQ96QV6
• Gene ID221613;
• SDS-PAGE MW 14kd
• Concentration 1.0mg/ml
• Formulation Rabbit Antiserum, containing 0.02% sodium azide as Preservative and 50% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:1000~1:2000
  

Histone H2A is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells.Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template.

• Western blot analysis of extracts from Hela cells, untreated (-) or treated with Nocodazole (1μg/ml, 24 hr; +), using #12095 diluted at 1:2,000.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting