• Product NamePLCgamma1(Phospho-Tyr783) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of PLCg1 only when phosphorylated at tyrosine 783.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of tyrosine 783 (G-F-Y(p)-V-E) derived from Human PLCG1.
• Target NamePLCgamma1
• ModificationPhospho
• Alternative Names    Phosphoinositide phospholipase C; Phospholipase C-gamma-1;
• Accession No. Swiss-Prot: P19174
  NCBI Protein: NP_002651.2
• UniprotP19174
• Gene ID5335;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 155kd
  Western blotting: 1:500~1:1000
   

PLC-gamma is a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase.

DeBell KE, et al. Mol Cell Biol. 1999 Nov; 19(11): 7388-7398.
Ver

• Western blot analysis of extracts from HL60 cells untreated or treated with EGF using PLCg1(Phospho-Tyr783) Antibody #11103.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting