protein_function: Tyrosine-protein kinase that acts as a cell-surfacereceptor for VEGFA, VEGFB and PGF, and plays an essential role inthe development of embryonic vasculature, the regulation ofangiogenesis, cell survival, cell migration, macrophage function,chemotaxis, and cancer cell invasion. May play an essential roleas a negative regulator of embryonic angiogenesis by inhibitingexcessive proliferation of endothelial cells. Can promoteendothelial cell proliferation, survival and angiogenesis inadulthood. Its function in promoting cell proliferation seems tobe cell-type specific. Promotes PGF-mediated proliferation ofendothelial cells, proliferation of some types of cancer cells,but does not promote proliferation of normal fibroblasts (invitro). Has very high affinity for VEGFA and relatively lowprotein kinase activity; may function as a negative regulator ofVEGFA signaling by limiting the amount of free VEGFA andpreventing its binding to KDR. Likewise, isoforms lacking atransmembrane domain, such as isoform 2, isoform 3 and isoform 4,may function as decoy receptors for VEGFA. Modulates KDR signalingby forming heterodimers with KDR. Ligand binding leads to theactivation of several signaling cascades. Activation of PLCG leadsto the production of the cellular signaling moleculesdiacylglycerol and inositol 1,4,5-trisphosphate and the activationof protein kinase C. Mediates phosphorylation of PIK3R1, theregulatory subunit of phosphatidylinositol 3-kinase, leading toactivation of phosphatidylinositol kinase and the downstreamsignaling pathway. Mediates activation of MAPK1,ERK2, MAPK3,ERK1and the MAP kinase signaling pathway, as well as of the AKT1signaling pathway. Phosphorylates SRC and YES1, and may alsophosphorylate CBL. Isoform 1 phosphorylates PLCG. Promotesphosphorylation of AKT1 at "Ser-473". Promotes phosphorylation ofPTK2,FAK1. Isoform 7 has a truncated kinase domain; it increasesphosphorylation of SRC at "Tyr-418" by unknown means and promotestumor cell invasion..
sVEGFR1, also known as sFMS-related tyrosine kinase 1(sFLT1). Oncogene sFLT belongs to the src gene family and is related to oncogene ROS . Like other members of this family, it shows tyrosine protein kinase activity that is important for the control of cell proliferation and differentiation. sFLT is mapped to 13q12. sVEGF receptor 1 signaling is essential for osteoclast development and bone marrow formation in colony-stimulating factor 1-deficient mice. The standard product used in this kit is recombinant human sVEGFR1, consisting of 905 amino acids with the molecular mass of 100KDa.
