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Mouse BAFF ELISA Kit

EK5268
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EK5268 96T $599.00

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Description
  • Product Name Mouse BAFF ELISA Kit
  • Specificity Mouse
  • Crossing Reactivity There is no detectable cross-reactivity with other relevant proteins.
  • Immunogen Type NSO,A127-L309
  • Alternative Names Tumor necrosis factor (Ligand) superfamily, member 13b; Tumor necrosis factor (Ligand) superfamily, member 13b, isoform CRA_d; Tnfsf13b; mCG_3400;
  • Accession No. Q3KP92
Application Details

sensitivity:10pg ml
Detect Range:46.9pg ml-3000pg ml
sample_type:cell culture supernates cell lysates tissue homogenates serum and plasma (heparin EDTA).
capture_antibody:monoclonal antibody from rat
detection_antibody:polyclonal antibody from goat
gene_name:TNFSF13B
protein_name:Tumor necrosis factor ligand superfamily member 13B
gene_full_name:tumor necrosis factor (ligand) superfamily member 13b
tissue_specificity:
sequence_similarities:
tmb_incubation:15-20min
research_category:immunology|adaptive immunity|b cells|non-cd|innate immunity|cytokines|tnf superfamily|signal transduction|growth factors/hormones|tnf|cancer|growth factors|kits/ lysates/ other|kits|elisa kits|tnf elisa kits

Product Description
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse BAFF
Background
protein_function:
B-cell activating factor(BAFF) also known as tumor necrosis factor ligand superfamily member 13B is a protein that in humans is encoded by the TNFLSF13B gene.1,2 BAFF is a cytokine that belongs to the tumor necrosis factor(TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B,TACI, TNFRSF17,BCMA, and TNFRSF13C,BAFFR. This cytokine is expressed in B cell lineage cells, and acts as a potent B cell activator. It has been also shown to play an important role in the proliferation and differentiation of B cells.3All these ligands act as heterotrimers(i.e. three of the same molecule) interacting with heterotrimeric receptors4, although BAFF has been known to be active as either a hetero- or homotrimer.5 The standard used in this kit is recombinant mouse soluble BAFF(A127-L309) with molecular weight 23.2KDa.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
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    • RbRabbit
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