• Product NameTau(Phospho-Ser214) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of Tau only when phosphorylated at serine 214.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of serine 214 (T-P-S(p)-L-P) derived from Human Tau.
• Target NameTau
• ModificationPhospho
• Alternative Names    MAPT; MTAPT; MTBT1; Neurofibrillary tangle protein; PHF-tau
• Accession No. Swiss-Prot: P10636
  NCBI Protein: NP _001116538.1
• UniprotP10636
• Gene ID4137;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 48 62 78 kd
  Western blotting: 1:500~1:1000
  

Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.

Smet C, et al. (2005) FEBS Lett; 579(19): 4159-64.
Puig B, et al. (2005) Acta Neuropathol (Berl).
Gvtz J, et al. (2001) Science; 293(5534): 1491-5.
Illenberger S, et al. (1998) Mol Biol Cell; 9(6): 1495-512.

• Western blot analysis of extracts from mouse brain tissue using Tau(Phospho-Ser214) Antibody #11109 and the same antibody preincubated with blocking peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting