• Product NameHuman HSP27 ELISA Kit
• SpecificityHuman
• Crossing ReactivityThere is no detectable cross-reactivity with other relevant proteins.
• Immunogen TypeE.coli,T2-K205
• Alternative Names    Heat shock protein beta-1; HspB1; 28 kDa heat shock protein; Estrogen-regulated 24 kDa protein; Heat shock 27 kDa protein; HSP 27; Stress-responsive protein 27; SRP27; HSPB1; HSP27, HSP28;
• Accession No. P04792
• UniprotP04792
• Gene ID3315;
• Cell Localization Cytoplasm. Nucleus. Cytoplasm, cytoskeleton,spindle. Cytoplasmic in interphase cells. Colocalizes withmitotic spindles in mitotic cells. Translocates to the nucleusduring heat shock and resides in sub-nuclear structures known asSC35 speckles or nuclear splicing speckles.

• sensitivity:5pg ml
  Detect Range:62.5pg ml-4000pg ml
  sample_type:cell culture supernates cell lysates serum plasma(heparin EDTA) and tissue homogenates.
  capture_antibody:monoclonal antibody from mouse
  detection_antibody:polyclonal antibody from goat
  gene_name:HSPB1
  protein_name:Heat shock protein beta-1
  gene_full_name:Heat shock protein beta-1
  tissue_specificity: Detected in all tissues tested: skeletalmuscle heart aorta large intestine small intestine stomach esophagus bladder adrenal gland thyroid pancreas testis adipose tissue kidney liver spleen cerebral cortex bloodserum and cerebrospinal fluid. Highest levels are found in theheart and in tissues composed of striated and smooth muscle..
  sequence_similarities:Belongs to the small heat shock protein (HSP20) family.
  tmb_incubation:25-30min
  research_category:HSPB1

• Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human HSP27

protein_function: Involved in stress resistance and actin organization.
Hsp27 is a chaperone of the sHsp(small heat shock protein) group among ubiquitin, alpha-crystallin, Hsp20 and others. The common functions of sHsps are chaperone activity, thermotolerance, inhibition of apoptosis, regulation of cell development, and cell differentiation. They also take part in signal transduction. The HSP27 gene has 3 exons. The mouse Hsp25 gene was mapped to chromosome 5 in a region homologous to 7q in the human. They also mapped the mouse Hsp105 gene to chromosome 5 but suggested that the human homolog is probably on 13q, not chromosome 7. The standard used in this kit is recombinant human HSP27, consisting of 205 amino acids with the molecular mass of 22.7kDa.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting