• Product NameNGEF Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification
• ApplicationsWB, IHC
• Species ReactivityHu, Ms, Rt
• SpecificityThe antibody detects endogenous levels of total NGEF protein.
• Immunogen TypePeptide
• Immunogen DescriptionFusion protein of human NGEF
• Target NameNGEF
• Alternative Names    EPHEXIN; ARHGEF27
• Accession No. Swiss-Prot#:Q8N5V2
  NCBI Gene ID:25791
  Gene Accssion:BC031573
• UniprotQ8N5V2
• Gene ID25791;
• Calculated MW 82 kDa
• Concentration 0.6
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• WB dilution:1:500-2000
  IHC dilution:1: 30-150
  

Acts as a guanine nucleotide exchange factor (GEF) which differentially activates the GTPases RHOA, RAC1 and CDC42. Plays a role in axon guidance regulating ephrin-induced growth cone collapse and dendritic spine morphogenesis. Upon activation by ephrin through EPHA4, the GEF activity switches toward RHOA resulting in its activation. Activated RHOA promotes cone retraction at the expense of RAC1- and CDC42-stimulated growth cone extension (By similarity).

• Gel: 6%SDS-PAGE, Lysate: 40 μg, Lane 1-2: Rat brain tissue and Mouse brain tissue lysates, Primary antibody:47316(NGEF Antibody) at dilution 1/250, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds
• The image is immunohistochemistry of paraffin-embedded Human tonsil tissue using 47316(NGEF Antibody) at dilution 1/35.(Original magnification: 200)
• The image is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 47316(NGEF Antibody) at dilution 1/35.(Original magnification: 200)

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting