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DNA-PKcs/PRKDC Antibody

48089
Review: comment rank 5 pdf
  Catalog No. Package Size Price
48089-1 50ul $260.00
48089-2 100ul $390.00

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Description
  • Product Name DNA-PKcs/PRKDC Antibody
  • Host Species Mouse
  • Clonality Monoclonal
  • Clone No. 2-B3
  • Purification ProA affinity purified
  • Applications WB, ICC, IHC
  • Species Reactivity Hu, Ms
  • Immunogen Description recombinant protein
  • Alternative Names DNA dependent protein kinase catalytic subunit antibody
    DNA PK catalytic subunit antibody
    DNA-dependent protein kinase catalytic subunit antibody
    DNA-PK catalytic subunit antibody
    DNA-PKcs antibody
    DNAPK antibody
    DNAPK catalytic subunit antibody
    DNPK 1 antibody
    DNPK1 antibody
    Hyper radiosensitivity of murine scid mutation, complementing 1 antibody
    Hyperradiosensitivity complementing 1, mouse, homolog of 1 antibody
    HYRC 1 antibody
    HYRC antibody
    HYRC1 antibody
    IMD26 antibody
    p350 antibody
    p460 antibody
    PKRDC antibody
    PRKDC antibody
    PRKDC_HUMAN antibody
    Protein Kinase DNA Activated Catalytic Polypeptide antibody
    XRCC 7 antibody
    XRCC7 antibody
  • Accession No. Swiss-Prot#:P78527
  • Calculated MW 469
  • Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
  • Storage Store at -20˚C
Application Details

WB: 1:1,000
IHC: 1:200
ICC: 1:100

Images
  • DNA-PKcs/PRKDC Antibody - SAB | Signalway Antibody

    Western blot analysis on HepG2 cell lysates using anti-DNA-PKcs Mouse mAb (Cat. # M1204-6).

  • DNA-PKcs/PRKDC Antibody - SAB | Signalway Antibody

    Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using anti-DNA-PKcs Mouse mAb (Cat.# M1204-6)

  • DNA-PKcs/PRKDC Antibody - SAB | Signalway Antibody

    Immunofluorescent staining of HepG2 cells using anti-DNA-PKcs Mouse mAb (Cat. # M1204-6).

Background
DNA-activated serine/threonine protein kinase (DNA-PK) is a heterotrimer of PRKDC and the Ku p70-p86 (XRCC6-XRCC5) dimer. DNA-PK is a nuclear protein kinase that is involved in DNA nonhomologous end joining (NHEJ) required for double- strand brake repair and V(D)J recombination.
Regerences
1."Frameshift mutation in PRKDC, the gene for DNA-PKcs, in the DNA repair-defective, human, glioma-derived cell line M059J."Anderson C.W., Dunn J.J., Freimuth P.I., Galloway A.M., Allalunis-Turner M.J.Radiat. Res. 156:2-9(2001)
2."Human DNA-activated protein kinase (DNA-PK) is homologous to phosphatidylinositol kinases."Poltoratsky V.P., Shi X., York J.D., Lieber M.R., Carter T.H.J. Immunol. 155:4529-4533(1995)
    Please let us know if you have published research using #48089 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  
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