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NeuN Antibody

48629
Review: comment rank 5 pdf
  Catalog No. Package Size Price
48629-1 50ul $260.00
48629-2 100ul $390.00

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Description
  • Product Name NeuN Antibody
  • Host Species Rabbit
  • Clonality Monoclonal
  • Clone No. SR45-07
  • Purification ProA affinity purified
  • Applications WB, ICC/IF, IHC, FC
  • Species Reactivity Hu, Ms, Rt
  • Immunogen Description recombinant protein
  • Alternative Names FLJ56884 antibody
    FLJ58356 antibody
    Fox-1 homolog C antibody
    fox1 homolog C antibody
    Fox3 antibody
    FOX3NeuN antibody
    hexaribonucleotide binding protein 3 antibody
    HRNBP3 antibody
    NEUN antibody
    neuronal nuclei antibody
    Rbfox3 antibody
    RFOX3_HUMAN antibody
    RNA binding protein fox-1 homolog 3 antibody
    RNA binding protein, fox 1 homolog (C. elegans) 3 antibody
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  • Accession No. Swiss-Prot#:A6NFN3
  • Calculated MW 34 kDa
  • Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
  • Storage Store at -20˚C
Application Details

WB: 1:1,000-1:2,000
IHC: 1:200-1:500
ICC: 1:50-1:200

FC: 1:50-1:100

Images
  • NeuN Antibody - SAB | Signalway Antibody

    Western blot analysis of NeuN on human brain lysates using anti-NeuN antibody at 1/1,000 dilution.( NeuN is expressed as two alternate transcripts with apparent SDS-PAGE molecular weight 46 and 48kDa)

  • NeuN Antibody - SAB | Signalway Antibody

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-NeuN antibody. Counter stained with hematoxylin.

  • NeuN Antibody - SAB | Signalway Antibody

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-NeuN antibody. Counter stained with hematoxylin.

  • NeuN Antibody - SAB | Signalway Antibody

    ICC staining NeuN in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • NeuN Antibody - SAB | Signalway Antibody

    Flow cytometric analysis of SH-SY-5Y cells with NeuN antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Background
Neuronal nuclei (NeuN, Fox-3, RBFOX3) is a nuclear protein expressed in most post-mitotic neurons of the central and peripheral nervous systems. NeuN is not detected in Purkinje cells, sympathetic ganglion cells, Cajal-Retzius cells, INL retinal cells, inferior olivary, and dentate nucleus neurons. This neuronal protein was originally identified by immunoreactivity with a monoclonal antibody also called NeuN. Using MS-analysis, NeuN was later identified as the Fox-3 gene product. Fox-3 contains an RNA recognition motif and functions as a splicing regulator. Fox-3 regulates alternative splicing of NumB, promoting neuronal differentiation during development.
Regerences
1. Patel TP et al. Single-neuron NMDA receptor phenotype influences neuronal rewiring and reintegration following traumatic injury. J Neurosci 34:4200-13 (2014).
2. Kaur P et al. Expression profiling of RNA transcripts during neuronal maturation and ischemic injury. PLoS One 9:e103525 (2014).
    Please let us know if you have published research using #48629 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
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