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Histone H2A(acetyl K9) Antibody

HW219
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  Catalog No. Package Size Price
HW219-1 50ul $260.00
HW219-2 100ul $390.00

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Description
  • Product Name Histone H2A(acetyl K9) Antibody
  • Host Species Rabbit
  • Clonality Monoclonal
  • Clone No. SR4-15
  • Purification ProA affinity purified
  • Applications WB, ICC/IF, IHC
  • Species Reactivity Hu, Ms, Rt
  • Immunogen Description recombinant protein
  • Alternative Names H2A antibody
    H2A1B_HUMAN antibody
    H2AFM antibody
    HIST1H2A antibody
    HIST1H2AE antibody
    Histone H2A type 1-B/E antibody
    Histone H2A.2 antibody
    Histone H2A/a antibody
    Histone H2A/m antibody
  • Accession No. Swiss-Prot#:P04908
  • Calculated MW 14 kDa
  • Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
  • Storage Store at -20˚C
Application Details

WB: 1:500-1:1,000
IHC: 1:50-1:200
ICC: 1:50-1:200

Images
  • Histone H2A(acetyl K9) Antibody - SAB | Signalway Antibody

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Histone H2A(acetyl K9) antibody. Counter stained with hematoxylin.

  • Histone H2A(acetyl K9) Antibody - SAB | Signalway Antibody

    Immunohistochemical analysis of paraffin-embedded mouse thyroid tissue using anti-Histone H2A(acetyl K9) antibody. Counter stained with hematoxylin.

  • Histone H2A(acetyl K9) Antibody - SAB | Signalway Antibody

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Histone H2A(acetyl K9) antibody. Counter stained with hematoxylin.

  • Histone H2A(acetyl K9) Antibody - SAB | Signalway Antibody

    Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-Histone H2A(acetyl K9) antibody. Counter stained with hematoxylin.

  • Histone H2A(acetyl K9) Antibody - SAB | Signalway Antibody

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Histone H2A(acetyl K9) antibody. Counter stained with hematoxylin.

  • Histone H2A(acetyl K9) Antibody - SAB | Signalway Antibody

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Histone H2A(acetyl K9) antibody. Counter stained with hematoxylin.

  • Histone H2A(acetyl K9) Antibody - SAB | Signalway Antibody

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Histone H2A(acetyl K9) antibody. Counter stained with hematoxylin.

  • Histone H2A(acetyl K9) Antibody - SAB | Signalway Antibody

    ICC staining Histone H2A(acetyl K9) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Histone H2A(acetyl K9) Antibody - SAB | Signalway Antibody

    ICC staining Histone H2A(acetyl K9) in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Histone H2A(acetyl K9) Antibody - SAB | Signalway Antibody

    ICC staining Histone H2A(acetyl K9) in MCF-7 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Background
Eukaryotic histones are basic and water soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene, that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation.
Regerences
1. Zhou, W. et al. 2013. Characterization of nuclear localization signal in the N terminus of integrin-linked kinase-associated phosphatase (ILKAP) and its essential role in the down-regulation of RSK2 protein signaling. J. Biol. Chem. 288: 6259-6271.
2. Chen, H. et al. 2013. Systems proteomics of healthy and diseased chromatin. Methods Mol. Biol. 1005: 77-93.
    Please let us know if you have published research using #HW219 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  
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