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Maltose Binding Protein Antibody

48666
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  Catalog No. Package Size Price
48666-1 50ul $260.00
48666-2 100ul $390.00

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Description
  • Product Name Maltose Binding Protein Antibody
  • Clone No. SR41-04
  • Purification ProA affinity purified
  • Applications WB
  • Species Reactivity Escherichia coli
  • Immunogen Description recombinant protein
  • Alternative Names ECK4026 antibody
    JW3994 antibody
    Mal E antibody
    MalE antibody
    malJ antibody
    Maltodextrin binding protein antibody
    Maltose ABC transporter periplasmic protein antibody
    Maltose binding periplasmic protein antibody
    MMBP antibody
    Periplasmic maltose binding protein antibody
  • Accession No. Swiss-Prot#:P0AEX9 P0AEY0
  • Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
  • Storage Store at -20˚C
Application Details

WB: 1:1,000-5,000

 

Images
  • Maltose Binding Protein Antibody - SAB | Signalway Antibody

    Western blot analysis of Maltose Binding Protein on recombinant MBP-tag protein lysates using anti-Maltose Binding Protein antibody at 1/1,000 dilution.

Background

Plasmid vectors for the expression of coding regions of eukaryotic genes in bacterial, insect and mammalian hosts are in common usage; such expression vectors frequently encode hybrid fusion proteins consisting in part of prokaryotic and in part, eukaryotic specified proteins. One such system utilizes maltose binding protein (MBP), the 370 amino acid product of the E. coli mal E gene. Plasmid vectors have been constructed utilizing the MBP domain that allow the synthesis of high levels of MBP-fusion proteins that can be purified in a one step procedure by affinity chromatography cross linked amylose resin. Once bound to amylose, the MBP protein can then be separated from the target protein by cleavage by coagulation factor Xa at a specific four residue site. Alternatively, the intact fusion protein can be specifically eluted from the resin by the addition of excess free maltose. Subsequent to elution, MBP fusion protein can be visualized either by Western blot analysis or immunoprecipitation using antibodies specific for the MBP-tag. Expression systems utilizing the MBP fusion tag include pCG-806fx and pMal vectors.

Regerences

1. Ishida K et al. Immunolocalization of anti-hsf1 to the acetabular glands of infectious schistosomes suggests a non-transcriptional function for this transcriptional activator. PLoS Negl Trop Dis 8:e3051 (2014).
2. Beck J et al. Ubiquitylation-dependent localization of PLK1 in mitosis. Nat Cell Biol 15:430-9 (2013).

    Please let us know if you have published research using #48666 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  
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