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C/EBP Beta Antibody

49008
Review: comment rank 5 pdf
  Catalog No. Package Size Price
49008-1 50ul $260.00
49008-2 100ul $390.00

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Description
  • Product Name C/EBP Beta Antibody
  • Host Species Rabbit
  • Clonality Monoclonal
  • Clone No. SC55-05
  • Purification ProA affinity purified
  • Applications WB, ICC/IF, IP, FC
  • Species Reactivity Hu, Ms, Rt
  • Immunogen Description recombinant protein
  • Alternative Names AGP/EBP antibody
    C EBP beta antibody
    C/EBP beta antibody
    C/EBP related protein 2 antibody
    CCAAT Enhancer Binding Protein beta antibody
    CCAAT/enhancer-binding protein beta antibody
    CEBPB antibody
    CEBPB_HUMAN antibody
    CRP2 antibody
    IL 6DBP antibody
    IL6DBP antibody
    Interleukin 6 dependent binding protein antibody
    LAP antibody
    Liver activator protein antibody
    Liver enriched transcriptional activator antibody
    NF IL6 antibody
    NFIL6 antibody
    Nuclear factor NF IL6 antibody
    Nuclear factor NF-IL6 antibody
    SF B antibody
    SFB antibody
    Silencer factor B antibody
    TCF-5 antibody
    TCF5 antibody
    Transcription factor 5 antibody
  • Accession No. Swiss-Prot#:P17676
  • Calculated MW 36 kDa
  • Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
  • Storage Store at -20˚C
Application Details

WB: 1:1,000-1:2,000

ICC: 1:50-1:200

FC: 1:50-1:100

Images
  • C/EBP Beta Antibody - SAB | Signalway Antibody

    Western blot analysis of C/EBP Beta on different lysates using anti-C/EBP Beta antibody at 1/1,000 dilution. Positive control:
    Lane 1: Hela
    Lane 2: PC-12

  • C/EBP Beta Antibody - SAB | Signalway Antibody

    ICC staining C/EBP Beta in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • C/EBP Beta Antibody - SAB | Signalway Antibody

    ICC staining C/EBP Beta in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • C/EBP Beta Antibody - SAB | Signalway Antibody

    ICC staining C/EBP Beta in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • C/EBP Beta Antibody - SAB | Signalway Antibody

    Flow cytometric analysis of Hela cells with C/EBP Beta antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Background
CCAAT-enhancer binding proteins (C/EBP) are basic region/leucine zipper (bZIP) transcription factors selectively expressed during the differentiation of liver, adipose tissue, blood cells and the endocrine pancreas. C/EBP β is a member of the C/EBP transcription factor family. The C/EBP β gene encodes several isoforms containing truncated transcription activation domains due to the alternative translational initiation at multipe AUG start sites. C/EBP β is also known as interleukin 6-dependent DNA-binding protein (IL6DBP), liver activator protein (LAP) or liver-enriched transcriptional activator protein transcription factor 5 (TCF5). C/EBP β contributes to the regulation of the acute phase response in hepatocytes. Stat3 has an important function in IL-6-mediated transcription of the C/EBP β gene that has direct implication for acute phase response in liver cells.The C/EBP β form requires phosphorylation for its DNA binding ability, and increase binding of C/EBP β isoforms during acute-phase reaction occurs through its upregulation and structural modification.
Regerences
1. Viart V et al. Transcription factors and miRNAs that regulate fetal to adult CFTR expression change are new targets for cystic fibrosis. Eur Respir J 45:116-28 (2015).
2. Xu S et al. LIFRa-CT3 induces differentiation of a human acute myelogenous leukemia cell line HL-60 by suppressing miR-155 expression through the JAK/STAT Pathway. Leuk Res N/A:N/A (2014).
    Please let us know if you have published research using #49008 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  
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