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COX1/Cyclooxygenase 1 Antibody

49017
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  Catalog No. Package Size Price
49017-1 50ul $260.00
49017-2 100ul $390.00

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Description
  • Product Name COX1/Cyclooxygenase 1 Antibody
  • Clone No. SC68-05
  • Purification ProA affinity purified
  • Applications WB, ICC, IHC, FC, IP
  • Species Reactivity Hu, Ms, Rt
  • Immunogen Description recombinant protein
  • Alternative Names COX 1 antibody
    COX 3 antibody
    COX-1 antibody
    COX1 antibody
    Cox3 antibody
    Cyclooxygenase 1 antibody
    Cyclooxygenase 3, included antibody
    Cyclooxygenase-1 antibody
    EC 1.14.99.1 antibody
    Partial COX1 proteins, included antibody
    PCOX1 antibody
    PGG/HS antibody
    PGH synthase 1 antibody
    PGH1_HUMAN antibody
    PGHS-1 antibody
    PGHS1 antibody
    PHS 1 antibody
    PHS1 antibody
    Prostaglandin G/H synthase 1 antibody
    Prostaglandin H2 synthase 1 antibody
    Prostaglandin-endoperoxide synthase 1 (prostaglandin G/H synthase and cyclooxygenase) antibody
    Prostaglandin-endoperoxide synthase 1 antibody
    PTGHS antibody
    PTGS1 antibody
  • Accession No. Swiss-Prot#:P23219
  • Calculated MW 69 kDa
  • Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
  • Storage Store at -20˚C
Application Details

WB: 1:1,000-5,000
IHC: 1:50-1:200
ICC: 1:100-1:500

FC: 1:50-1:100

Images
  • COX1/Cyclooxygenase 1 Antibody - SAB | Signalway Antibody

    Flow cytometric analysis of Hela cells with COX1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

  • COX1/Cyclooxygenase 1 Antibody - SAB | Signalway Antibody

    ICC staining COX1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • COX1/Cyclooxygenase 1 Antibody - SAB | Signalway Antibody

    ICC staining COX1 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • COX1/Cyclooxygenase 1 Antibody - SAB | Signalway Antibody

    ICC staining COX1 in C2C12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • COX1/Cyclooxygenase 1 Antibody - SAB | Signalway Antibody

    ICC staining COX1 in L6 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • COX1/Cyclooxygenase 1 Antibody - SAB | Signalway Antibody

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-COX1 antibody. Counter stained with hematoxylin.

  • COX1/Cyclooxygenase 1 Antibody - SAB | Signalway Antibody

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-COX1 antibody. Counter stained with hematoxylin.

  • COX1/Cyclooxygenase 1 Antibody - SAB | Signalway Antibody

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-COX1 antibody. Counter stained with hematoxylin.

  • COX1/Cyclooxygenase 1 Antibody - SAB | Signalway Antibody

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-COX1 antibody. Counter stained with hematoxylin.

  • COX1/Cyclooxygenase 1 Antibody - SAB | Signalway Antibody

    Western blot analysis of COX1 on different lysates using anti-COX1 antibody at 1/1,000 dilution. Positive control:
    Lane 1: C2C12
    Lane 2: A431

Background

Cytochrome c oxidase subunit I, COX1 (also designated COI, MTCO1 or oxidative phosphorylation (OxPhos) Complex IV, subunit I) is one of three mitochondrial DNA (mtDNA) encoded subunits (MTCO1-3) of respiratory Complex IV. Cytochrome c oxidase is a hetero-oligomeric enzyme composed of 13 subunits localized to the mitochondrial inner membrane and is the terminal enzyme complex of the electron transport chain. Complex IV catalyzes the reduction of molecular oxygen to water. The energy released is used to transport protons across the mitochondrial inner membrane. The resulting electro-chemical gradient is necessary for the synthesis of ATP. Complex IV contains 13 polypeptides; COX1, COX2 and COX3 (MTCO1-3) make up the catalytic core and are encoded by mtDNA while subunits IV, Va, Vb, VIa, VIb, VIc, VIIa, VIIb, VIIc and VIII are nuclear-encoded.

Regerences

1. Cui TZ et al. Modulation of the respiratory supercomplexes in yeast: enhanced formation of cytochrome oxidase increases the stability and abundance of respiratory supercomplexes. J Biol Chem 289:6133-41 (2014).

    Please let us know if you have published research using #49017 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
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